Skip to Content
MilliporeSigma
All Photos(2)

Key Documents

ABT61

Sigma-Aldrich

Anti-INF2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Inverted formin-2, HBEBP2-binding protein C

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_071934

UniProt accession no.

Q27J81

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... INF2(64423)

General description

INF2 (inverted formin 2) is a mouse formin that has been found to interact with actin through the C-terminus to the formin homology 2 domain. The WASP homology 2 motif activates another region that appropriates actin monomers. INF2 has a unique ability to speed up polymerization and depolymerization of actin filaments. Mutations in the N-terminal diaphanous inhibitory domain of INF2 have been proven to be a main cause of familial focal segmental glomerulosclerosis.

Specificity

Other homologies: Mouse (68% sequence homology).

Immunogen

KLH-conjugated linear peptide corresponding to human INF2.

Application

Anti-INF2 Antibody is an antibody against INF2 for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot of this antibody detected INF2 in stomach cancer tissue.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected INF2 in 10 µg of HeLa cell lysate.

Target description

~155 kDa observed.
INF2 has been shown in WB to migrate as an ~155 kDa band (Madrid, R., et al. (2010). Dev Cell. 18(5):814-827).
Uniprot describes 3 isoforms produced by alternative splicing with MW′s @ ~135, ~134 & ~25 kDa

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Katherine Young Bezold Lamm et al.
eLife, 7 (2018-01-09)
Healthy pregnancy depends on proper placentation-including proliferation, differentiation, and invasion of trophoblast cells-which, if impaired, causes placental ischemia resulting in intrauterine growth restriction and preeclampsia. Mechanisms regulating trophoblast invasion, however, are unknown. We report that reduction of Inverted formin 2

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service