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Key Documents

ABT331

Sigma-Aldrich

Anti-Nephrin Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Nephrin, NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

human (immunogen homology)

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

O60500

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NPHS1(4868)

General description

Nephrin (NPHS1, NPHN), also known as is Renal glomerulus-specific cell adhesion receptor, is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.

Immunogen

KLH-conjugated linear peptide corresponding to human Nephrin.

Application

Detect Nephrin using this rabbit polyclonal antibody, Anti-Nephrin Antibody validated for use in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 1 µg/mL from a representative lot detected Nephrin in mouse kidney tissue.

Immunofluorescence Analysis: 10 µg/mL from a representative lot detected Nephrin in mouse kidney tissue.

Quality

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Nephrin in 15 µg of mouse kidney tissue lysate.

Target description

~100 kDa observed. Uncharacterized bands may appear in some lysate(s). Despite its calculated molecular weight of 135 kDa, Nephrin often migrates at a lower than expected size in SDS-PAGE.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Feng Xu et al.
Annals of translational medicine, 8(18), 1154-1154 (2020-11-27)
Diabetic nephropathy (DN) is one of the common chronic microvascular complications of diabetes, and podocyte injury and dysfunction are strictly related to the pathogenesis of DN. Studies have shown that ligustilide (LIG) has anti-inflammatory, antioxidant, and anti-apoptotic activities. This study

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