ABT1384

Anti-Neurocan Antibody, N-terminal

from rabbit

• Synonym(s): Neurocan core protein, 245 kDa early postnatal core glycoprotein, Chondroitin sulfate proteoglycan 3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: unpurified
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, rat
• packaging: antibody small pack of 25 μL
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; radioimmunoassay: suitable
• isotype: IgG
• NCBI accession no.: NP_113841 (neurocan core protein precursor)
• UniProt accession no.: P55067
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: rat ... Ncan(58982)

Description

General description

Neurocan core protein (UniProt: P55067; also known as 245 kDa early postnatal core glycoprotein, Chondroitin sulfate proteoglycan 3) is encoded by the Ncan (also known as Cspg3) gene in rat. Neurocan is a secreted protein of the aggrecan/versican proteoglycan family that is found in early postnatal and adult brain. During early postnatal development neurocan accounts for at least 20% of the protein of the soluble brain proteoglycans. Neurocan mostly contains chondroitin sulfate, but also has N-linked and O-linked oligosaccharides. It is shown to be involved in the modulation of cell adhesion and migration and regulates neurite outgrowth. Neurocan interact with several cell surface molecules, such as N-CAM, L1/Ng-CAM, etc., and modulates the cell-binding and neurite outgrowth promoting activates of these molecules. Neurocan is synthesized with a 22 aa signal peptide that is subsequently cleaved to produce the mature form. Neurocan contains one C-type lectin domain, two EGF-like domains, one Ig-like V type domain, two link domains and one Sushi (CCP/SCR) domain. (Ref.: Rauch, U., et al. (2001) Cell Mol. Life Sci. 58(12-13):1842-56; Zhou, XH et al. (2001). Mol. Cell Biol. 21(17): 5970-78).

Immunogen

GST-tagged recombinant fragement corresponding to 164 amino acids from the N-terminal region of rat Neurocan.

Application

Anti-Neurocan, N-terminal, Cat. No. ABT1384, is a rabbit polyclonal antibody that detects Neurocan core protein and has been tested for use in Immunofluorescence, Immunohistochemistry, and Radioimmunoassay.

Radioimmunoassay Analysis: A representative lot detected Neurocan in rat brain (Milev, P., et. al. (1998). Biochem Biophys Res Commun. 247(2):207-12).

Immunohistochemistry Analysis: A representative lot detected Neurocan in E13 and E14 mouse ventral diencephalon and retina in C57 mouse embryos (Leung, K.M., et. al. (2004). Brain Res Dev Brain Res. 152(1):1-10).

Quality

Evaluated by Immunofluorescence in E14 Embryonic rat brain (frozen section), rat E14 neocortex, and rat hippocampus cells.

Immunofluorescence Analysis: A 1:500 dilution of this antibody detected Neurocan in E14 Embryonic rat brain (frozen section), rat E14 neocortex, and rat hippocampus cells.

Target description

135.55 kDa calculated.

Physical form

Format: Unpurified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1