ABS994
Anti-phospho-Glucocorticoid receptor Antibody, Ser226
from rabbit, purified by affinity chromatography
Synonym(s):
Nuclear receptor subfamily 3 group C member 1, glucocorticoid receptor, GR
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer226)
Gene Information
human ... NR3C1(2908)
General description
Glucocorticoid receptor (GR), also known as Nuclear receptor subfamily 3 group C member 1, and encoded by the gene name NR3C1/ GRL, is receptor for glucocorticoids (GC). GR has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. GR affects inflammatory responses, cellular proliferation and differentiation in target tissues. Recent studies have shown that GR may act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. In addition, GR is involved in chromatin remodeling, and may play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression. Mutation in GR has been associated with Glucocorticoid resistance (GCRES): a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. GR is widely expressed. In the heart, GR is detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node, as well as whole adult and fetal heart.
Immunogen
Epitope: A region within the modulating domain
KLH-conjugated linear peptide corresponding to the domain of: Modulating region of Human Glucocorticoid receptor.
Application
Research Category
Signaling
Signaling
Research Sub Category
Lipid Metabolism & Weight Regulation
Lipid Metabolism & Weight Regulation
This Anti-phospho-Glucocorticoid receptor antibody is validated for use in Western Blotting for the detection of phospho-Glucocorticoid receptor.
Western Blotting Analysis: A representative lot of this antibody at 0.5 µg/mL dilution detected phosphorylated glucocorticoid receptor protein in transfected U2OS cell lysate (Chen, W., et al. Mol Endocrinol, August 2008, 22(8):1754–1766)
Western Blotting Analysis: A representative lot of this antibody at 0.5 µg/mL dilution detected phosphorylated glucocorticoid receptor protein in rat hepatoma cell lysate (Blind,R., et al. Journal of Steroid Biochemistry & Molecular Biology 109 (2008) 150–157)
Western Blotting Analysis: A representative lot of this antibody at 0.5 µg/mL dilution detected phosphorylated glucocorticoid receptor protein in rat hepatoma cell lysate (Blind,R., et al. Journal of Steroid Biochemistry & Molecular Biology 109 (2008) 150–157)
Quality
Evaluated by Western Blotting on transfected HEK293 cell lysate
Western Blotting Analysis: 1 µg/mL dilution of this antibody detected phosphorylated glucocorticoid receptor in 6 µg of transfected HEK293 cell lysate
Western Blotting Analysis: 1 µg/mL dilution of this antibody detected phosphorylated glucocorticoid receptor in 6 µg of transfected HEK293 cell lysate
Target description
~85 kDa observed
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Glucocorticoid receptor phosphorylation differentially affects target gene expression.
Chen, W; Dang, T; Blind, RD; Wang, Z; Cavasotto, CN; Hittelman, AB; Rogatsky, I; Logan et al.
Molecular Endocrinology null
Differential recruitment of glucocorticoid receptor phospho-isoforms to glucocorticoid-induced genes.
Blind, RD; Garabedian, MJ
The Journal of Steroid Biochemistry and Molecular Biology null
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