ABC1414

Anti-phospho IGPR-1 (Ser220)

• Synonym(s): Transmembrane and immunoglobulin domain-containing protein 2;CD28 homolog;Immunoglobulin and proline-rich receptor 1
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• mol wt: calculated mol wt 30.68 kDa; observed mol wt ~45 to 55 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 100 μg
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_001162597.1
• UniProt accession no.: Q96BF3
• storage temp.: -10 to -25°C
• target post-translational modification: phosphorylation (pSer220)
• Gene Information: human ... TMIGD2(126259)

Description

General description

Transmembrane and immunoglobulin domain-containing protein 2 (UniProt: Q96BF3; also known as CD28 homolog, Immunoglobulin and proline-rich receptor 1, IGPR-1) is encoded by the TMIGD2 (also known as CD28H, IGPR1, UNQ3059/PRO9879) gene (Gene ID: 126259) in human. IGPR-1 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 23-150), a transmembrane domain (aa 151-171), and a cytoplasmic domain (aa 172-282). It is widely expressed with higher expression observed in epithelial and endothelial cells, including bronchial epithelial cells of lung, breast glandular and lobular epithelia cells. It plays a role in cell-cell interaction, cell migration, and angiogenesis. IGPR-1 is localized to endothelial adherens junctions, and through trans-homophilic dimerization it regulates endothelial cell-cell adhesion and barrier function. This dimerization stimulates phosphorylation of serine 220, which is required for IGPR-1 to regulate endothelial barrier function and angiogenesis. Through interaction with HHLA2, IGPR-1 is reported to co-stimulate T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an Akt-dependent signaling cascade. IGPR-1 is also phosphorylated on serine 220 by I B kinase β, which leads to its activation. Activated IGPR-1 then stimulates phosphorylation of AMPK, which in turn phosphorylates ULK1 and Beclin-1 and leads to increased levels of LC3-II. Genotoxic agents such as doxorubicin and flow shear stress are also reported to simulate phosphorylation of IGPR-1 at serine 220. (Ref.: Amraei, R., et al. (2020). J. Biol. Chem. 295(49); 16691-16699; Wang, YHW., et al.(2016). J. Mol. Biol. 428(24; PtB); 5019-5033).

Specificity

This rabbit polyclonal antibody detects Immunoglobulin and proline-rich receptor 1 (IGPR-1) phosphorylated on serine 220.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids surrounding phosphoserine 220 in the cytoplasmic domain in Human Immunoglobulin and proline-rich receptor 1 (IGPR-1).

Application

Qualtiy Control Testing

Evaluated by Western Blotting in lysate from HEK293 cells overexpressing IGPR-1.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected IGPR-1 phosphorylated on serine 220 in lysates from HEK293 cells overexpressing IGPR-1.

Tested applications

Immunofluorescence Analysis: A representative lot detected phospho-IGPR-1 (Ser220) in Immunofluorescence applications (Wang, Y., et. al. (2016). J Mol Biol. 428(24 Pt B):5019-5033).

Immunohistochemistry Applications: A representative lot detected phospho-IGPR-1 (Ser220) in Immunohistochemistry applications (Wang, Y., et. al. (2016). J Mol Biol. 428(24 Pt B):5019-5033).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Physical form

Purified rabbit polyclonal antibody in PBS without azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable