AB1920
Anti-Integrin α3 Antibody
serum, Chemicon®
Synonym(s):
CD49c
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
porcine, rat, goat, canine, sheep, hamster, equine, pig, bovine, human, mouse, chicken, horse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ITGA3(3675)
General description
Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 19 alpha and 8 beta subunits that form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. Both of the subunits contribute to the binding of the ligand. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.
Specificity
Integrin alpha3. Specificity determined by immunoprecipitation from a detergent extract of 35S-methionine labeled neuroblastoma cells. No cross-reactivity to alpha1, alpha2, alpha4, alpha5, alpha6, or alphaV integrins.
Reactivity with other species has not been determined.
Immunogen
A synthetic peptide (CRIQPSETERLTDDY) derived from the COOH terminal sequence located in the cytoplasmic domain of the human alpha3 integrin subunit.
Application
Detect Integrin α3 using this Anti-Integrin α3 Antibody validated for use in ELISA, IF, IH, IP, RIA & WB.
ELISA/RIA:
A 1:1,000 dilution of a previous lot was used in Enzyme-linked Immunosorbent Assay/Radioimmunoassay.
Immunoprecipitation:
5 µL of a previous lot precipitated alpha3/beta1 from 5 x 106 cells (Sherry, D.M., 2001).
Immunohistochemistry/immunofluorescence:
1:500 dilution of a previous lot was used, suggest use on acetone-fixed tissue.
Western Blot Analysis:
1:500 dilution of a previous lot was used.
Optimal working dilutions must be determined by end user.
A 1:1,000 dilution of a previous lot was used in Enzyme-linked Immunosorbent Assay/Radioimmunoassay.
Immunoprecipitation:
5 µL of a previous lot precipitated alpha3/beta1 from 5 x 106 cells (Sherry, D.M., 2001).
Immunohistochemistry/immunofluorescence:
1:500 dilution of a previous lot was used, suggest use on acetone-fixed tissue.
Western Blot Analysis:
1:500 dilution of a previous lot was used.
Optimal working dilutions must be determined by end user.
Quality
Routinely evaluated by Western Blot on A431 lysates.
Western blot: 1:500 dilution of this lot detected INTEGRIN ALPHA 3 on 10 μg of A431 lysates.
Western blot: 1:500 dilution of this lot detected INTEGRIN ALPHA 3 on 10 μg of A431 lysates.
Target description
130 kDa
Linkage
Replaces: AB1948
Physical form
Rabbit polyclonal IgG antiserum containing 0.05% sodium azide.
Analysis Note
Control
Human fetal foreskin fibroblasts (HFFF), neuroblastomas
Human fetal foreskin fibroblasts (HFFF), neuroblastomas
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The role of a recombinant fragment of laminin-332 in integrin alpha3beta1-dependent cell binding, spreading and migration.
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Thomas C Sroka et al.
Carcinogenesis, 27(9), 1748-1757 (2006-03-16)
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Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 20(12), 1101-1109 (2001-01-11)
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