AB1413-I
Anti-Interleukin-1β
from rabbit, purified by affinity chromatography
Synonym(s):
Interleukin-1 beta, IL-1 beta, IL-1b, IL-1beta
Sign Into View Organizational & Contract Pricing
All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
species reactivity (predicted by homology)
gerbil (based on 100% sequence homology), hamster (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
gerbil ... Il1B(110551400)
hamster ... Il1B(100775079)
mouse ... Il1B(16176)
rat ... Il1B(24494)
General description
Interleukin-1 beta (UniProt; also known as IL-1 beta, IL-1b, IL-1beta) is encoded by the Il1b gene (Gene ID 16176) in murine species. Interleukin-1 (IL-1) is a cytokine that plays a central role in the regulation of acute and chronic inflammation. Two forms of IL-1, IL-1 and IL-1 , are transcribed from two separate genes and mediate similar, but not completely overlapping, biological effects via the membrane-bound IL-1 type 1 receptor (IL-1R1). Studies utilizing Il1a- and Il1b-knockout mice showed that IL-1 , but not IL-1 , is involved in T-cell-dependent antibody production via antigen-specific T-cell activation. IL-1 binding to the extracellular domain of IL1R1 promotes the recruitment of the IL-1 receptor accessory protein and subsequent signal transduction mediated by the cytoplasmic domains of IL-1R1 and IL1-R2. The IL-1 type 2 receptor (IL1R2) is unable to initiate signaling and only acts as a decoy receptor. Soluble receptors for IL-1 (sIL-1Rs) are produced by proteolytic cleavage of membrane-bound receptors or by alternative splicing and act as natural inhibitors by competing against membrane-bound IL1Rs for IL-1 binding. Murine IL-1 beta is produced with a propeptide sequence (a.a. 1-117), which is removed posttranslationally to yield the mature (a.a. 118-269) protein.
Specificity
This rabbit polyclonal antibody detected both the ~17 kDa mature and ~30 kDa pro-form of murine IL-1beta. The corresponding sequence in human IL-1beta differs from that of the immunogen by only one amino acid. Immunogen sequence is not found in IL-1alpha.
Immunogen
KLH-conjugated linear peptide corresponding to a region within the C-terminal half of the mature murine and rat IL-1beta sequence.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
This rabbit polyclonal Anti-Interleukin-1 Antibody, Cat. No. AB1413-I is validated for use in Western Blotting for the detection of Interleukin-1 .
Western Blotting Analysis: 2 µg/mL from a representative lot detected pro- and mature IL-1beta in 10 µg of lysates from mouse testis tissue and LPS-stimulated RAW264.7 cells, respectively.
Quality
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected IL-1beta in 10 µg of NIH/3T3 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected IL-1beta in 10 µg of NIH/3T3 cell lysate.
Target description
~17/30 kDa observed. 17.39/30.93 kDa (mouse mature/pro-form), 17.32/30.64 kDa (rat mature/pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).
Linkage
Replaces: AB1413
Physical form
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Jaehyun Bae et al.
Scientific reports, 12(1), 13595-13595 (2022-08-11)
The α-glucosidase inhibitor (α-GI) delays the intestinal absorption of glucose, which reduces postprandial hepatic glucose intake. This mechanism is considered to be effective in treating non-alcoholic fatty liver disease (NAFLD). Here, we investigated the effect of voglibose, an α-glucosidase inhibitor
Qianyu Zhang et al.
Frontiers in nutrition, 9, 854655-854655 (2022-07-16)
Radiological therapy/examination is the primary source of artificial radiation exposure in humans. While its application has contributed to major advances in disease diagnosis and treatment, ionizing radiation exposure is associated with ovarian damage. The use of natural products, either alone
Mohammad Harun-Or-Rashid et al.
Journal of neuroinflammation, 15(1), 313-313 (2018-11-15)
Glaucoma is a chronic degenerative disease for which inflammation is considered to play a pivotal role in the pathogenesis and progression. In this study, we examined the impact of a ketogenic diet on the inflammation evident in glaucoma as a
Muhammad M Hossain et al.
Cells, 11(12) (2022-06-25)
Endoplasmic reticulum (ER) stress and neuroinflammation are involved in the pathogenesis of many neurodegenerative disorders. Previously, we reported that exposure to pyrethroid insecticide deltamethrin causes hippocampal ER stress apoptosis, a reduction in neurogenesis, and learning deficits in adult male mice.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service