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Key Documents

AB12226

Sigma-Aldrich

Anti-RALY Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse)), RNA-binding protein (autoantigenic), hnRNP associated with lethal yellow protein homolog, hnRNP core protein C-like 2, RNA-binding protein (autoantigenic, hnRNP-ass

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human, rat

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_057951

UniProt accession no.

Q9UKM9

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RALY(22913)

General description

RNA-binding protein RALY is a member of the RRM HNRPC family and contains a characteristic RRM (RNA recognition motif) domain. It is thought to participate in pre-mRNA splicing and may be a heterogenous nuclear ribonucleoprotein. Phosphorylation of RALY has been observed following damage to DNA. Expression has been observed in brain, heart, kidney, liver, lung, pancreas, and skeletal muscle.

Immunogen

Histidine-tagged recombinant protein corresponding to human RALY.

Application

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated RALY from HepG2 cell lysate.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
Use Anti-RALY Antibody (rabbit polyclonal antibody) validated in WB, IP to detect RALY also known as hnRNP core protein C-like 2, RNA-binding protein (autoantigenic hnRNP-associated with lethal yellow).

Quality

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 1:10,000 dilution of this antibody detected RALY on 10 µg of HepG2 cell lysate.

Target description

~ 38 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HepG2 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Emma Bondy-Chorney et al.
Cell reports, 33(4), 108318-108318 (2020-10-29)
Polyphosphates (polyPs) are long chains of inorganic phosphates linked by phosphoanhydride bonds. They are found in all kingdoms of life, playing roles in cell growth, infection, and blood coagulation. Unlike in bacteria and lower eukaryotes, the mammalian enzymes responsible for

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