426205

β-Lactamase, Bacillus cereus 569/H9

• Synonym(s): β-Lactamase, Bacillus cereus 569/H9, Penicillinase
• CAS Number: 9073-60-3
• Enzyme Commission number: 3.5.2.6 (BRENDA, IUBMB)
• MDL number: MFCD00131819
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• biological source: Bacillus sp. (Bacillus cereus)
• Quality Level: 100
• form: lyophilized
• packaging: vial of ≥50 units β-lactamase II; vial of ≥500 units β-lactamase I
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: sterile distilled water: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

β-Lactamase produced by bacteria shares sequence homology to penicillin-binding proteins. This enzyme is found in Gram-negative bacteria. β-Lactamases have four molecular classes, named A, B, C, and D. A, C, and D form an acyl-enzyme via active site serine residue. Class B β-lactamases are metalloenzymes, which have a zinc ion at their active site for β-lactam hydrolysis.

Biochem/physiol Actions

β-Lactamase hydrolyzes β-lactum antibiotics and is the chief cause of resistance to β-lactam antibiotics development by bacteria. Mutations in the β-lactamases are associated with extended-spectrum β-lactamases (ESBLs).

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol benzyl penicillin and 1.0 µmol of cephalosporin C, respectively, per min at 25°C.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 month at 4°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable