368059-M

Granzyme B Substrate II, Fluorogenic

A fluorogenic substrate for the detection of granzyme B and caspase-8 activity.

• Synonym(s): Granzyme B Substrate II, Fluorogenic, Z-IETD-AFC, Caspase-8 Substrate II
• Empirical Formula (Hill Notation): C₃₇H₄₂F₃N₅O₁₃
• Molecular Weight: 821.75
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• assay: ≥97% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: white
• solubility: DMSO: 1 mg/mL
• fluorescence: λ_ex ~400 nm; λ_em ~505 nm
• storage temp.: −20°C

Description

General description

A fluorogenic substrate for the detection of granzyme B and caspase-8 activity. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

A fluorogenic substrate for the detection of granzyme B. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).

Biochem/physiol Actions

Primary Target
Fluorogenic substrate for the detection of granzyme B and caspase-8 activity

Warning

Toxicity: Standard Handling (A)

Sequence

Z-Ile-Glu-Thr-Asp-AFC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Karahashi, H., and Amano, F. 2000. Biol. Pharm. Bull. 23, 140.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable