344220

Formyl Peptide Receptor-Like 1 Antagonist, WRW⁴

The Formyl Peptide Receptor-Like 1 Antagonist, WRW⁴ controls the biological activity of Formyl Peptide Receptor-Like 1.

• Synonym(s): Formyl Peptide Receptor-Like 1 Antagonist, WRW⁴, H₂N-WRWWWW-CONH₂, FPRL1 Antagonist, WRW⁴
• Empirical Formula (Hill Notation): C₆₁H₆₅N₁₅O₆
• Molecular Weight: 1104.27
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• color: white
• solubility: DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A Trp-rich hexapeptide that acts as a potent and specific antagonist of Formyl Peptide Receptor-Like 1 (FPRL1)-mediated signaling. Shown to bind to FPRL1 and interfere with ligand-receptor interaction {IC₅₀ = 230 nM for FPRL1 Agonist, WKYMVm (Cat. No. 344216)}. Completely blocks ligand-mediated [Ca²⁺]_i increase, ERK activation, and chemotactic migration of FPRL1-expressing RBL-2H3 cells at a concentration of 10 µM. Also inhibits Aβ₄₂ peptide-induced superoxide generation and prevents its internalization in human macrophages.

A Trp-rich hexapeptide that acts as a potent and specific antagonist of Formyl Peptide Receptor-Like 1 (FPRL1)-mediated signaling. Shown to bind to FPRL1 and interfere with the ligand-receptor interaction {IC₅₀ = 230 nM for FPRL1 Agonist, WKYMVm (Cat. No. 344216)}. Completely blocks the ligand activated [Ca²⁺]_i increase, ERK activation and chemotactic migration of FPRL1-expressing RBL-2H3 cells at 10 µM. Further, inhibits Aβ₄₂ peptide-induced superoxide generation and prevents its internalization in human macrophages.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Formyl Peptide Receptor-Like 1 (FPRL1)

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 230 nM for FPRL1 Agonist

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Trp-Arg-Trp-Trp-Trp-Trp-CONH₂

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Bae, Y.S., et al. 2004. J. Immunol.173, 607.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable