324412

Dynamin Inhibitor III, Phthaladyn-23

• Synonym(s): Dynamin Inhibitor III, Phthaladyn-23, 4-Chloro-2-((2-(3-nitrophenyl)-1,3-dioxo-2,3-dihydro-1H-isoindole-5-carbonyl)-amino)-benzoic acid
• Empirical Formula (Hill Notation): C₂₂H₁₂ClN₃O₇
• Molecular Weight: 465.80
• UNSPSC Code: 12352200
• NACRES: NA.28

Properties

• assay: ≥98% (HPLC)
• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: light yellow to beige
• solubility: DMSO: 7 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A cell-permeable phthalimide compound that is reported to inhibit dynamin GTPase activity (IC₅₀ = 17.4 and 63 µM using purified sheep brain dynamin I and recombinant dynamin II, respectively) in a GTP-competitive manner. Shown to block K⁺-induced SVE (synaptic vesicle endocytosis) of FM4-64 dye into rat brain synaptosomes (IC₅₀ = 12.9 µM), while being ineffective at 30 µM against RME (receptor-mediated endocytosis) of transferrin into human osteosarcoma epithelial U2OS cells, presumably due to poor dynamin II inhibitory potency and/or rapid efflux and/or limited permeability in this cell line.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Odell, L.R., et al. 2010. J. Med. Chem.53, 5267.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable