219415

Cathepsin Inhibitor I

The Cathepsin Inhibitor I controls the biological activity of Cathepsin. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

• Synonym(s): Cathepsin Inhibitor I, Z-FG-NHO-Bz, CATI -1
• Empirical Formula (Hill Notation): C₂₆H₂₅N₃O₆
• Molecular Weight: 475.49
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: >90% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: white
• solubility: DMSO: soluble; acetonitrile: soluble; ethanol: soluble
• shipped in: ambient
• storage temp.: 10-30°C

Description

General description

Cell-permeable cysteine protease inhibitor. Inhibits cathepsin B (k₂/K_i = 8.9 x 10³ M^-1 sec^-1), cathepsin L (k₂/K_i = 3.8 x 10⁵ M^-1 sec^-1), cathepsin S (k₂/K_i = 4.2 x 10⁴ M^-1 sec^-1), and papain (k₂/K_i = 2.4 x 10³ M^-1 sec^-1).

Cysteine protease inhibitor. Selectively inhibits cathepsin B (k₂/K_i = 8.9 x 10³ M^-1sec^-1), cathepsin L (k₂/K_i = 3.8 x 10⁵ M^-1sec^-1), cathepsin S (k₂/K_i = 4.2 x 10⁴ M^-1sec^-1), and papain (k₂/K_i = 2.4 x 10³ M^-1sec^-1). Half-life in buffered solution (pH 5-9) = 25 h (30°C); 300 h (0°C).

Half-life in buffered solution (pH 5-9) = 25 h (30°C); 300 h (0°C).

Biochem/physiol Actions

Cell permeable: yes

Primary Target
cathepsin B

Product does not compete with ATP.

Reversible: no

k₂/K_i = 8.9 x 10³ M⁻¹sec⁻¹ for cathepsin B

Warning

Toxicity: Standard Handling (A)

Sequence

Z-Phe-Gly-NHO-Bz

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Zhu, D. and Uckun, F.M. 2000. Clin. Cancer Res.6, 2064.
Demuth, H.-U., et al. 1996. Biochim. Biophys. Acta 1295, 179.
Brömme, D., and Demuth, H.-U. 1994. Methods Enzymol. 244, 671.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable