196810

Bax-Inhibiting Peptide, V5

A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection.

• Synonym(s): Bax-Inhibiting Peptide, V5, BIP-V5, H-VPMLK-OH
• Empirical Formula (Hill Notation): C₂₇H₅₀N₆O₆S
• Molecular Weight: 586.79
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: white
• solubility: water: 1 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection. Functions as effectively as the Caspase Inhibitor VI (Z-VAD-FMK; Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also effectively blocks caspase-independent necrotic cell death. Shown to be Ku70 competitive, interact with Bax, prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days). Negative control peptide is also available (Cat. No. 196811).

A cell-permeable pentapeptide derived from the Ku70-Bax inhibiting domain that has been shown to offer protection against apoptosis in cells of human, mouse, rant, and porcine origin. Reported to function as effectively as the caspase inhibitor, Z-VAD-FMK (Caspase Inhibitor VI, Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also reported to effectively block caspase-independent necrotic cell death. Shown to interact with Bax in a Ku70-competitive manner and prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days).

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Bax mediated apoptosis

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 50-200 µM against Bax mediated apoptosis

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Val-Pro-Met-Leu-Lys-OH

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Storage in glass vials is not recommended as loss of activity may occur.

Other Notes

Yoshida, T., et al. 2004. Biochem. Biophys. Res. Commun.321, 961.
Sawada, M., et al. 2003. Nature Cell Biol.5, 352.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable