14-919-M

LRRK2 Protein, active, 10 µg

Active, N-terminal FLAG-tagged, recombinant, human LRRK2, amino acids 970-end. For use in Kinase Assays.

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100; 300
• recombinant: expressed in baculovirus infected Sf21 cells
• mol wt: M_w 180 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• GenBank accession no.: BC117180
• UniProt accession no.: Q5S007
• Gene Information: human ... LRRK2(120892)

Description

General description

Product Source: N-terminal FLAG-tagged, recombinant, human LRRK2 amino acids 970-end, expressed by baculovirus in Sf21 insect cells.

This protein is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.

Packaging

Also available in 250μg size as catalog # 14-919M and in bulk as catalog # 14-919-K.

Quality

Routinely evaluated by phosphorylation of ERMtide (RLGRDKYKTLRQIRQ)

Physical form

50mM Tris/HCl pH7.4, 300mM NaCl, 20mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 150µg/ml FLAG peptide, 20% v/v glycerol, 1mM benzamidine, 0.2mM PMSF, 5mM DTT. Frozen solution.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3