126575
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free
Synonym(s):
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free
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About This Item
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assay
≥95% (cellulose acetate electrophoresis)
form
powder
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤0.02% fatty acids
≤3% ash
≤5% moisture
color
white to light yellow
light yellow to light brown
shipped in
ambient
storage temp.
2-8°C
General description
Albumin, Bovine Serum, (BSA) Fraction V, Fatty Acid-Free is useful for immunodiagnostic assays. It is designed for use in serological testing, radioimmunoassay, and hormone response studies. It is also applicable in binding and transport studies. BSA is prepared by cold ethanol extraction (Cohn method) with a pH: 6.8-7.4.
Bovine serum albumin (BSA) is a non-glycosylated globular, α-helical protein belonging to the serum albumin family. It consists of three domains with two sub-domains each and has 17-disulfide bonds.
Application
Albumin, Bovine Serum, Fraction V, Fatty Acid-Free has been used:
- as a component of: blocking buffer immunoelectron microscopy (IEM) for various cells
- mitochondrial isolation buffer (MIB) and mitochondria assay solution (MAS) to trap free fatty acids released during adipose tissue homogenization
- blocking buffer for wholemount staining of the esophageal epithelium (EE) in immunofluorescence
Biochem/physiol Actions
Bovine Serum Albumin (BSA), the most vital component of cell culture media, aids in embryonic stem cells (hESC) differentiation. It aids the transportation of drugs, hormones, and fatty acids. BSA is also used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
Warning
Toxicity: Standard Handling (A)
Preparation Note
May take up to 24 h to fully dissolve.
Reconstitution
Following reconstitution, store in the refrigerator (4°C). 0.1% NaN₃ may be added as a preservative. Stock solutions are stable for up to 3 months at 4°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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