1.03857
GranuCult® prime Levine Eosin-Methylene Blue (L-EMB) Agar
ISO 21150, acc. FDA-BAM
Synonym(s):
EMB agar, LEVINE, Eosin Methylene-blue Lactose Agar acc. to LEVINE
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About This Item
UNSPSC Code:
41106213
NACRES:
NA.74
Recommended Products
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ISO 21150
Quality Level
form
powder
technique(s)
microbiological culture: suitable
pH
7.1±0.2 (25 °C)
application(s)
food and beverages
pharmaceutical
storage temp.
15-25°C
suitability
selective and differential for enterobacteriaceae
Related Categories
General description
GranuCult® prime Eosin Methylene Blue (L-EMB) Agar is a nutrient rich medium which is designed for the isolation, confirmation, and identification of Escherichia coli, and other Enterobacteriaceae from the food chain, cosmetic and other materials.
The synergistic effect of lactose and two indicator dyes, Eosin Y and Methylene Blue differentiates colonies of lactose fermenting and non-fermenting microbes. Growing colonies of E. coli produce a green metallic sheen due to high acid production from lactose. Other coliforms do not produce enough acid to attain the metallic sheen. The growing coliforms produce blue-black colonies due to the formation of eosin-methylene blue dye complex when the pH drops.
Peptones in the medium provide nitrogen, amino acids, and minerals to support growth. Di-potassium phosphate acts as a buffer and agar is the solidifying agent.
The synergistic effect of lactose and two indicator dyes, Eosin Y and Methylene Blue differentiates colonies of lactose fermenting and non-fermenting microbes. Growing colonies of E. coli produce a green metallic sheen due to high acid production from lactose. Other coliforms do not produce enough acid to attain the metallic sheen. The growing coliforms produce blue-black colonies due to the formation of eosin-methylene blue dye complex when the pH drops.
Peptones in the medium provide nitrogen, amino acids, and minerals to support growth. Di-potassium phosphate acts as a buffer and agar is the solidifying agent.
Application
GranuCult® prime Eosin Methylene Blue (L-EMB) Agar is used for the isolation, confirmation, and identification of Escherichia coli , and other Enterobacteriaceae from the food chain, cosmetic and other materials.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
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Warning
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Hazard Classifications
Skin Sens. 1
Storage Class
11 - Combustible Solids
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WGK 2
Certificates of Analysis (COA)
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