1.01398

Malachite green oxalate (C.I.42000)

for microscopy and for microbiology

• Synonym(s): Malachite green oxalate (C.I.42000)
• Empirical Formula (Hill Notation): C₅₂H₅₄N₄O₁₂
• CAS Number: 2437-29-8
• Molecular Weight: 927.00
• UNSPSC Code: 41116130
• EC Index Number: 219-441-7

Properties

• Quality Level: 400
• form: solid
• composition: Dye, ≥90% spectrophotometric assay
• pH: 2.4 (24 °C, 10 g/L in H₂O)
• mp: 159 °C
• solubility: 110 g/L
• bulk density: 400‑500 kg/m³
• εmax: 1730-1960 at 616-620 nm in water at 0.003 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/2C23H25N2.3C2H2O4/c2*1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3*3-1(4)2(5)6/h2*5-17H,1-4H3;3*(H,3,4)(H,5,6)/q2*+1;;;/p-2
• InChI key: CNYGFPPAGUCRIC-UHFFFAOYSA-L

Description

General description

Malachite green Oxalate (C.I. 42000) is a dry dye that is used for the preparation of a staining solution that can be used for a spore staining according to Rakette. It is possible to use Malachite green dye for differential spore staining in bacteriological samples of human origin, Dye is used in a hot staining method to stain specifically spores. Counterstain with a red dye for better visualization. The dye has a constant dye content and a reliable high quality and thus, is registered and certified as IVD & CE product, one of the major precondition for a reproducible diagnostic staining.

Analysis Note

Dye content (spectrophotometrically): ≥ 90% (als C₄₆H₅₀N₄ *2 C₂HO₄ *C₂H₂O₄)
Identity
- oxalate:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 616 - 620 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; water): 1730 - 1960
TLC-Test: passes test
Loss on drying (50 °C / <1 hPa): ≤ 7%
Suitability for microbiology: passes test
Suitability for microscopy: passes test

Safety Information

• pictograms: GHS05,GHS06,GHS08,GHS09
• signalword: Danger
• hcodes: H301 - H318 - H361d - H410
• pcodes: P201 - P202 - P273 - P280 - P301 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2
• Storage Class: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable