09-019
Anti-REST Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Neural-restrictive silencer factor, RE1-silencing transcription factor, X2 box repressor, neuron restrictive silencer factor, repressor binding to the X2 box
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... REST(5978)
mouse ... Rest(19712)
rat ... Rest(83618)
General description
REST (RE1-silencing transcription factor) also known as NRSF (neuron-restrictive silencer factor) is a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element). The binding of REST to the NRSE represses neuronal gene transcription in non-neuronal cells. Although REST is most highly expressed in non-neural tissues, it is also expressed in developing neurons and at low levels in the brain. REST contains nine zinc finger domains, but also exists as a C-terminally truncated form produced by alternative splicing. This variant, REST4, contains five of the zinc-finger domains and weakly binds DNA, yet is transported to the nucleus. REST associates with mSin3 and HDAC in ventricular myocytes, suggesting a role for REST outside the nervous system. Down-regulation of REST, which normally occurs upon neural differentiation, is necessary for the proper development of certain classes of neurons. REST is required to repress neuronal gene expression in vivo, in both extra-neural and undifferentiated neural tissue.
Specificity
Recognizes REST
Immunogen
Epitope: C-terminus
GST fusion protein corresponding to residues 801-1097 of human REST.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Signaling Neuroscience
Transcription Factors
Signaling Neuroscience
This Anti-REST Antibody is validated for use in WB for the detection of REST.
Quality
Evaluated by western blot with HeLa nuclear extract.
Western Blot Analysis:
0.5-1 μg/mL of this antibody detected REST in nuclear extracts from HeLa, Raji, and Jurkat cells.
Western Blot Analysis:
0.5-1 μg/mL of this antibody detected REST in nuclear extracts from HeLa, Raji, and Jurkat cells.
Target description
120 kDa
Physical form
Affinity purified rabbit serum in PBS with 0.07% sodium azide, and 30% glycerol.
Antigen Affinity Purified
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
HeLa nuclear extract
HeLa nuclear extract
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Loss of the repressor REST in uterine fibroids promotes aberrant G protein-coupled receptor 10 expression and activates mammalian target of rapamycin pathway.
Varghese, BV; Koohestani, F; McWilliams, M; Colvin, A; Gunewardena, S; Kinsey, WH; Nowak et al.
Proceedings of the National Academy of Sciences of the USA null
Pei-Ching Chang et al.
PloS one, 9(2), e88556-e88556 (2014-02-20)
Prostate cancer (PCa) cells undergoing neuroendocrine differentiation (NED) are clinically relevant to the development of relapsed castration-resistant PCa. Increasing evidences show that autophagy involves in the development of neuroendocrine (NE) tumors, including PCa. To clarify the effect of autophagy on
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