05-598

Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002

clone RR002, Upstate^®, from mouse

• Synonym(s): H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RR002, monoclonal
• species reactivity: vertebrates, nematode, Caenorhabditis elegans, human
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• suitability: not suitable for ELISA applications
• NCBI accession no.: NM_003493
• UniProt accession no.: Q16695
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer10)
• Gene Information: human ... HIST1H3F(8968)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Broad species cross-reactivity expected due to sequence conservation.

This antibody recognizes Histone H3 phosphorylated at Serine 10.

Immunogen

Peptide (ARK[pS]TGGKAPRKQL-C) corresponding to amino acids 7-20 of human histone H3 with a c-terminal cyteine added for conjugation purposes.

Application

Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002 is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as Anti-H3S10p, is published in peer reviewed journals and is validated in ICC, IHC, WB.

ELISA Analysis:
This antibody showed no immuno-reactivity with an unphosphorylated Histone H3 peptide (residues 7-20), nor with other histone phosphopeptides.

Immunohistochemistry:
A 1:1000 dilution of this antibody detected mitotic cells in C. elegans embryo sections as shown by an independent laboratory.

Immunocytochemistry:
This lot showed positive staining of mitotic cells in cultures of HeLa cells undergoing exponential growth

Quality

Routinely evaluated by western blot on acid extracted proteins from colcemid-arrested HeLa cells.

Western Blot Analysis:
0.005-0.025 μg/mL of this lot detected phosphorylated Histone H3 in acid extracted proteins from colcemid-arrested HeLa cells.

Target description

17 kDa

Physical form

Format: Purified

Purified mouse IgG in buffer containing 0.1M Tris Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Analysis Note

Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extrats.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1