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Key Documents

05-525

Sigma-Aldrich

Anti-Rad50 Antibody, clone 13B3/2C6

clone 13B3/2C6, Upstate®, from mouse

Synonym(s):

Anti-NBSLD, Anti-RAD502, Anti-hRad50

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

13B3/2C6, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_005732

UniProt accession no.

Q92878

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... RAD50(10111)
mouse ... Rad50(19360)

Specificity

Rad50

Immunogen

GST fusion protein corresponding to residues 1-425 of human Rad50

Application

Anti-Rad50 Antibody, clone 13B3/2C6 is a high quality Mouse Monoclonal Antibody for the detection of Rad50 & has been validated in IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

routinely evaluated by immunoblot on HeLa nuclear lysate

Target description

153kDa

Physical form

10mM PBS, pH 7.4
Format: Purified
Protein G Chromatography

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A tool for enhancement and scoring of DNA repair foci.
Gerashchenko, BI; Dynlacht, JR
Cytometry. Part A : the Journal of the International Society For Analytical Cytology null
K M Trujillo et al.
The Journal of biological chemistry, 273(34), 21447-21450 (1998-08-15)
Genetic studies in yeast have indicated a role of the RAD50 and MRE11 genes in homologous recombination, telomere length maintenance, and DNA repair processes. Here, we purify from nuclear extract of Raji cells a complex consisting of human Rad50, Mre11
Functional role for senataxin, defective in ataxia oculomotor apraxia type 2, in transcriptional regulation.
Suraweera, A; Lim, Y; Woods, R; Birrell, GW; Nasim, T; Becherel, OJ; Lavin, MF
Human Molecular Genetics null
Mutations in the MRE11, RAD50, XRS2, and MRE2 genes alter chromatin configuration at meiotic DNA double-stranded break sites in premeiotic and meiotic cells.
Ohta, K, et al.
Proceedings of the National Academy of Sciences of the USA, 95, 646-651 (1998)
G M Dolganov et al.
Molecular and cellular biology, 16(9), 4832-4841 (1996-09-01)
In this report, we describe the identification and molecular characterization of a human RAD50 homolog, hRAD50. hRAD50 was included in a collection of cDNAs which were isolated by a direct cDNA selection strategy focused on the chromosomal interval spanning 5q23
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