05-1951-S
Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1, Trial Size
clone 18E9.1, from mouse
Synonym(s):
H3K27me3, Histone H3 (trimethyl K27), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3
About This Item
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
18E9.1, monoclonal
species reactivity
human, mouse
technique(s)
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
trimethylation (Lys27)
Gene Information
human ... H3C1(8350)
mouse ... H3C1(360198)
General description
Specificity
Immunogen
Application
Epigenetics & Nuclear Function
Histones
Western Blot (Snap i.d.) Analysis: A 1:25,000 dilution from a representative lot detected Histone H3 in 10 µg of HeLa acid extract.
Immunocytochemistry Analysis: A 1:125 dilution from a representative lot detected Histone H3 in A431 and NIH/3T3 cells.
Dot Blot Analysis: Histone peptides with various modifications (see table) were transferred to
PVDF membrane and probed with Anti-Trimethyl Histone H3 (Lys4) (1:100 dilution). Proteins were visualized using a Donkey Anti-rabbit IgG conjugated to HRP and a chemiluminescence detection systems.
Quality
Western Blot Analysis: A 1:50,000 dilution of this antibody detected Histone H3 in 10 µg of HeLa acid extract.
Target description
Physical form
Storage and Stability
Analysis Note
HeLa acid extract
Disclaimer
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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