05-1517

Anti-hnRNP Q Antibody, clone 18E4

clone 18E4, from mouse

• Synonym(s): heterogeneous nuclear ribonucleoprotein Q-like, Synaptotagmin-binding, cytoplasmic RNA-interacting protein, Glycine- and tyrosine-rich RNA-binding protein, GRY-RBP, NS1-associated protein 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 18E4, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_001153145.1
• UniProt accession no.: O60506
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SYNCRIP(10492)

Description

General description

Heterogeneous nuclear ribonucleoprotein Q commoly refered to as hnRNP Q can be found as three different isoforms derived from alternative splicing of a single gene. This RNA binding protein is implicated in control of mRNA precursor splicing for the survival of motor neurons (SMN) protein, a gene. Loss-of-function mutations of SMN are linked to spinal muscular atrophy (SMA) a common neurodegenerative disease. It has been shown that hnRNP Q proteins interact with SMN, and this is interacton is required for efficient pre-mRNA splicing in vitro. Current data suggests that hnRNP Q is a splicing modulator of SMN.

Specificity

This antibody recognizes hnRNP Q.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to human hnRNP Q.

Application

Immunocytochemistry
Cited by independent researcher using a representative lot.
Immunoprecipitation
Cited by independent researcher using a representative lot.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-hnRNP Q Antibody, clone 18E4 (Mouse Monoclonal Antibody) validated in WB, ICC, IP to detect hnRNP Q also known as heterogeneous nuclear ribonucleoprotein Q-like.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected hnRNP Q on 10 µg of HeLa cell lysate.

Target description

The calculated molecular weight is 70 kDa Clone 18E4 can recognizes four bands on the Western Blot of total HeLa cell lysate corresponding to molecular masses of ~80, ~70, ~60 and ~55 kDa hnRNP Q has three isoforms of ~70– 55 kDa The ~80 kDa reactive band was identified as hnRNP R, which migrates at ~80 kDa on SDS–PAGE. (Mourelatos., Z., et al., EMBO J., 20, 5443-5452 (2001).)

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable