05-1312-I
Anti-Ubiquityl-Histone H2B Antibody, clone 56
clone 56, from mouse
Synonym(s):
Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, H2B/al, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l)
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All Photos(4)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
56, monoclonal
species reactivity
mouse, human, rat
packaging
antibody small pack of 25 μg
technique(s)
ChIP: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... HIST1H2BC(8347)
General description
Histone H2B type 1-C/E/F/G/I (UniProt: P62807; also known as Histone H2B.1 A, Histone H2B.a, H2B/a, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l) is encoded by the HIST1H2BC (also known as H2BFL, HIST1H2BE H2BFH, HIST1H2BF, H2BFG, HIST1H2BG, H2BFA, HIST1H2BI, H2BFK) gene (Gene ID: 3017, 8339, 8343, 8344, 8346, 8347) in human. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. Histone H2B is one of the core components of the nucleosome, the smallest subunit of chromatin. DNA base pairs are wrapped around an octamer of core histone proteins consisting of two each of Histone H2A, H2B, H3 and H34. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. A number of studies have implicated ubiquitinated H2B in heterochromatic gene silencing and in the control of gene expression. Histone H2B is ubiquitylated at Lys120 by RAD6 E2 protein in conjunction with RNF20/RNF40 and this ubiquitinylated form is found preferentially associated with the transcribed regions of highly expressed genes.
Specificity
Clone 56 recognizes histone H2B when ubiquitinated on Lys120. It does not recognize H2B in its unmodified state or ubiquitin unconjugated to H2B.
Immunogen
A branched peptide corresponding to the ubiquitination site on human H2B.
Application
Anti-Ubiquityl-Histone H2B, clone 56 Antibody, Cat. No. 05-1312-I, is a highly specific mouse monoclonal antibody that targets Ubiquityl-Histone H2B and has been tested in Chromatin Immunoprecipitation (ChIP) and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Western Blotting Analysis: A representative lot detected Ubiquityl-Histone H2B in HeLa, NIH3T3, and PC-12 cell lysate.
Western Blotting Analysis: A representative lot detected Ubiquityl-Histone H2B in U2OS whole cell extract, purified histone H2A, purified H2B, and HEK293 acid extracted proteins (Courtesy of Dr. Moshe Oren, Weizmann Institute of Science).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Ubiquityl-Histone H2B in uH2B and H3K4me2 (Courtesy of Dr. Moshe Oren, Weizmann Institute of Science).
Western Blotting Analysis: A representative lot detected Ubiquityl-Histone H2B in U2OS whole cell extract, purified histone H2A, purified H2B, and HEK293 acid extracted proteins (Courtesy of Dr. Moshe Oren, Weizmann Institute of Science).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Ubiquityl-Histone H2B in uH2B and H3K4me2 (Courtesy of Dr. Moshe Oren, Weizmann Institute of Science).
Quality
Evaluated by Western Blotting in PC-12 cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected Ubiquityl-Histone H2B in 10 µg of PC-12 cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected Ubiquityl-Histone H2B in 10 µg of PC-12 cell lysate.
Target description
~25 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Smaragda Fanourgakis et al.
Computational and structural biotechnology journal, 21, 614-629 (2023-01-21)
The timing and location of writing and erasing of histone modifications determine gene expression programs and are tightly controlled processes. One such modification is the monoubiquitination of histone H2B (H2Bub), whose precise level during transcription elongation is dynamically regulated by
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