03-0186-00

SMC^® Human IFN-α2 High Sensitivity Immunoassay Kit

• Synonym(s): IFN-alpha-2 Single Molecule Counting Kit, IFN-alpha-2 immunoassay kit, IFN-alpha-2 ultra-sensitive immunoassay, Interferon alpha-A immunoassay kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• species reactivity: canine, feline, porcine, rat, nonhuman primates
• manufacturer/tradename: SMC^®
• assay range: sensitivity: 0.062 pg/mL; (lower limit of quantification); inter-assay cv: <13%; intra-assay cv: <9%
• technique(s): ELISA: suitable; single molecule counting: suitable
• relevant disease(s): autoimmune diseases
• storage temp.: 2-8°C

Description

General description

Human Interferon alpha-2 (IFNa2) is a cytokine belonging to the IFN family exhibiting antiviral, antitumor and antiproliferative effects on immune response. IFNa2 is secreted by viral infected cells and acts to inhibit viral infection. It has been shown to play a role in autoimmune diseases such as lupus and myositis. The mechanisms of action underlying the pathogenic role that IFNa and IFNß play in autoimmunity is not well understood but elucidating their role will lead to a better understanding of these cytokines in chronic inflammation and immunity.

The SMC^® Human IFN-α2 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO^® immunoassay systems detect analytes using Single Molecule Counting (SMC^®) technology.

Specificity

Cross Reactivity:
This assay was tested for cross reactivity with rhIFN-β, rhIFN-γ, rhIFN-λ1 (IL-29), rhIFN-λ2 (IL28A), rhIFN-λ3 (IL-28B), rhIFN-ω, IFN-α2a, IFN-α2b and IFN-α.

• There is no cross-reactivity detected with rhIFN-ß, rhIFN-γ, rhIFN-λ1 (IL-29), rhIFN-λ2 (IL-28A),rhIFN-λ3 (IL-28B), and rhIFN-ω.
• There is 100% cross-reactivity with IFN-α2a.
• There is 49% cross-reactivity with IFN-α2b.
• There is 3% cross-reactivity with IFN-α.

Accuracy:

• Minimum of 5 serum samples spiked with average recovery of 102% with a range of 92-111%
• Minimum of 5 plasma samples spiked with average recovery of 96% with a range of 89-107%

Application

Research Category:

• Inflammation & Immunology

Research Sub-Category:

• Inflammatory Disease
• Immune Response
• Autoimmune disease

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC^® Human IFN-a2 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IFN-a2 in human serum and plasma.

Features and Benefits

This new streamline kit has the following features including:

• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO^® and Erenna

Components

• Assay Buffer
• IFN-a2 Coated Beads
• Standard Diluent
• IFN-a2 Detection Antibody
• IFN-a2 Standard
• 10X Wash Buffer
• Buffer D
• Elution Buffer B

Analysis Note

Studied using five normal plasma samples run in triplicate by 3 different operators on 3 different days.

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Although IFN-α2 was detectable in rat, pig, non-human primate, feline, and canine samples; the assay has not been validated for these species.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302 + H332,H311,H319,H360FD,H412
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects