WHA10400014
Whatman® nitrocellulose membrane filters
pore size 12 μm, diam. 50 mm, pack of 100 ea
Synonym(s):
Whatman filter, Z696927, cellulose membrane, membrane filter
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About This Item
UNSPSC Code:
40161507
NACRES:
NB.24
sterility
non-sterile
packaging
pack of 100 ea
manufacturer/tradename
Whatman 10400014
Whatman Article No. 28414921 (US reference)
diam.
50 mm
pore size
12 μm
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General description
Recommended for the majority of routine applications, this membrane is manufactured under strictly controlled conditions. The user will benefit from the performance improvements which are now available in Whatman membrane filters, including very narrow pore size distribution and low levels of extractables.
Higher strength and flexibility:
Most membranes are inherently brittle and difficult to handle; it is not uncommon for filters to be damaged during loading into holders or while in use. Whatman cellulose nitrate membrane filters have a noticeably improved flexibility and are made to tolerate abuse during handling, loading and autoclaving without sacrificing integrity. These membranes are among the strongest of their type available, as measured and compared by burst pressure tests.
Low extractable levels:
The level of extractables in membrane filters has become more important with advances in filtration or adsorption techniques. In particular, pharmaceutical, immunological and biomedical tissue culture and trace analysis applications can be adversely affected by high extractable levels. Whatman cellulose nitrate membrane filters have a low level of extractables, generally below that of other membranes of a similar type.
Narrow pore size distribution:
One of the major features of Whatman membrane filters is the narrow distribution of pore sizes. The rated pore size of these membranes is closely controlled due to the advanced manufacturing and control system. Additionally, the batch-to-batch variation is minimized providing more consistent laboratory results.
Increased temperature stability:
Membrane filters are normally autoclaved at 121°C without loss of integrity. Cellulose nitrate membranes are supplied as circles, sheets or reels.
Reduced shrinkage:
Excessive shrinkage can cause problems during autoclaving and is often the cause of membranes tearing in their holders after autoclaving. It may also cause a reduction in flow rate and total throughput. Whatman membranes exhibit a low shrinkage during autoclaving.
Applications:
Higher strength and flexibility:
Most membranes are inherently brittle and difficult to handle; it is not uncommon for filters to be damaged during loading into holders or while in use. Whatman cellulose nitrate membrane filters have a noticeably improved flexibility and are made to tolerate abuse during handling, loading and autoclaving without sacrificing integrity. These membranes are among the strongest of their type available, as measured and compared by burst pressure tests.
Low extractable levels:
The level of extractables in membrane filters has become more important with advances in filtration or adsorption techniques. In particular, pharmaceutical, immunological and biomedical tissue culture and trace analysis applications can be adversely affected by high extractable levels. Whatman cellulose nitrate membrane filters have a low level of extractables, generally below that of other membranes of a similar type.
Narrow pore size distribution:
One of the major features of Whatman membrane filters is the narrow distribution of pore sizes. The rated pore size of these membranes is closely controlled due to the advanced manufacturing and control system. Additionally, the batch-to-batch variation is minimized providing more consistent laboratory results.
Increased temperature stability:
Membrane filters are normally autoclaved at 121°C without loss of integrity. Cellulose nitrate membranes are supplied as circles, sheets or reels.
Reduced shrinkage:
Excessive shrinkage can cause problems during autoclaving and is often the cause of membranes tearing in their holders after autoclaving. It may also cause a reduction in flow rate and total throughput. Whatman membranes exhibit a low shrinkage during autoclaving.
Applications:
- Sample preparation
- Microbiological studies
- Filtration of aqueous solutions
Other Notes
Field of Use : For internal research use only. Products are not intended for diagnostic use or resale.
Legal Information
Whatman is a registered trademark of Cytiva
Certificates of Analysis (COA)
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Dori M Landry et al.
Genes & development, 23(23), 2753-2764 (2009-12-03)
Most eukaryotic mRNAs are translated using a cap-dependent mechanism of translation. However, approximately 10% of mammalian mRNAs initiate translation using a cap-independent mechanism that is not well understood. These mRNAs contain an internal ribosome entry site (IRES) located in the
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