QBD10200

NHS-dPEG^®₄-biotin

• Synonym(s): (+)-Biotin-PEO4-NHS-propionate, NHS-PEO4-Biotin, Polyethylene glycol
• Empirical Formula (Hill Notation): C₂₅H₄₀N₄O₁₀S
• CAS Number: 459426-22-3
• Molecular Weight: 588.67
• UNSPSC Code: 12161502
• PubChem Substance ID: 329823890
• NACRES: NA.22

Properties

• assay: >90%
• form: solid or viscous liquid
• reaction suitability: reagent type: cross-linking reagent; reaction type: Biotinylations; reactivity: amine reactive; reagent type: linker
• polymer architecture: shape: linear; functionality: monofunctional
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: O=C(ON1C(CCC1=O)=O)CCOCCOCCOCCOCCNC(CCCCC2C3NC(NC3CS2)=O)=O
• InChI: 1S/C25H40N4O10S/c30-20(4-2-1-3-19-24-18(17-40-19)27-25(34)28-24)26-8-10-36-12-14-38-16-15-37-13-11-35-9-7-23(33)39-29-21(31)5-6-22(29)32/h18-19,24H,1-17H2,(H,26,30)(H2,27,28,34)/t18-,19-,24-/m0/s1
• InChI key: DTLVBHCSSNJCMJ-JXQFQVJHSA-N

Description

General description

NHS-dPEG^®₄-biotin is a water-soluble cross-linking reagent used in biotinylation reactions. The N-hydroxysuccinimidyl (NHS) moiety of NHS-dPEG^®₄-biotin forms a stable amide bond with free primary amines, such as the amines on the side chain of lysine. The biotin moiety is used in a variety of applications, including labeling, supramolecular building, affinity chromatography, pull-down assays, and the production of biotinylated antibodies.

Features and Benefits

NHS-dPEG^®₄-biotin is used extensively to label molecules that contain a free amine with biotin. The single molecular weight NHS-dPEG^®₄-biotin has a long spacer of precise length (19.2 Å) between the biotin moiety and the labeled molecule. This dPEG^® spacer imparts a high degree of water solubility to the biotin label. For biotinylated peptides and proteins, the dPEG^® spacer dramatically reduces aggregation and precipitation of the modified molecule and reduces non-specific binding of the molecule, both of which are common problems with molecules that have been biotinylated with the widely used NHS-LC-biotin.

Legal Information

Products Protected under U.S. Patents # 7,888,536 B2

dPEG is a registered trademark of Quanta BioDesign

Safety Information

• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable