809705
5-DBCO-PEG4-dUTP, 10mM Aqueous Solution
Synonym(s):
5-DBCO-PEG4-dUTP
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
assay
>95% (HPLC)
Quality Level
form
solution
reaction suitability
reaction type: click chemistry
solubility
Tris buffer: 100 mM (pH 7.5)
shipped in
wet ice
storage temp.
−20°C
SMILES string
O=C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O2)O)C=C1/C=C\CNC(CCOCCOCCOCCOCCNC(CCC(N3CC4=CC=CC=C4C#CC5=CC=CC=C53)=O)=O)=O)=O
Application
5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service