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Exopolysaccharide Quantification for the Plant Pathogen Ralstonia solanacearum.

Bio-protocol (2017-05-20)
Rémi Peyraud, Timothy P Denny, Stéphane Genin
ABSTRAKT

Soluble exopolysaccharide is a major virulence factor produced by the plant pathogen Ralstonia solanacearum. Its massive production during plant infection is associated with the arrest of water flow in xylem vessels leading eventually to plant death. The composition of this heavy macromolecule includes mainly N-acetylgalactosamine. Here we describe a colorimetric method for quantitative determination of the soluble exopolysaccharide present in culture supernatant of R. solanacearum.

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Ammonium sulfate, for molecular biology, ≥99.0%
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L-Glutamic acid monosodium salt monohydrate, ≥98.0% (NT)
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Potassium phosphate monobasic, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
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