- Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.
Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.
Optics letters (1994-06-01)
S W Hell, J Wichmann
PMID19844443
ABSTRAKT
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation point-spread function. In contrast to near-field scanning optical microscopy, this method can produce three-dimensional images of translucent specimens.
MATERIAŁY
Numer produktu
Marka
Opis produktu
Sigma-Aldrich
Abberior® FLIP 565, maleimide, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
Sigma-Aldrich
Abberior® STAR 440SXP, maleimide, for long Stokes STED and 2-color STED application
Sigma-Aldrich
Abberior® STAR 440SXP, NHS ester, for long Stokes STED and 2-color STED application