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Structural guided scaffold phage display libraries as a source of bio-therapeutics.

PloS one (2013-08-21)
Y K Stella Man, Danielle DiCara, Nicole Chan, Sandrine Vessillier, Stephen J Mather, Michelle L Rowe, Mark J Howard, John F Marshall, Ahuva Nissim
ABSTRAKT

We have developed a structurally-guided scaffold phage display strategy for identification of ligand mimetic bio-therapeutics. As a proof of concept we used the ligand of integrin αvβ6, a tumour cell surface receptor and a major new target for imaging and therapy of many types of solid cancer. NMR structure analysis showed that RGD-helix structures are optimal for αvβ6 ligand-interaction, so we designed novel algorithms to generate human single chain fragment variable (scFv) libraries with synthetic VH-CDR3 encoding RGD-helix hairpins with helices of differing pitch, length and amino acid composition. Study of the lead scFv clones D25scFv and D34scFv and their corresponding VH-CDR3 derived peptides, D25p and D34p, demonstrated: specific binding to recombinant and cellular αvβ6; inhibition of αvβ6-dependent cell and ligand adhesion, αvβ6-dependent cell internalisation; and selective retention by αvβ6-expressing, but not αvβ6-negative, human xenografts. NMR analysis established that both the D25p and D34p retained RGD-helix structures confirming the success of the algorithm. In conclusion, scFv libraries can be engineered based on ligand structural motifs to increase the likelihood of developing powerful bio-therapeutics.

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Sigma-Aldrich
Anti-Integrin αVβ6 Antibody, clone 10D5, azide free, clone 10D5, Chemicon®, from mouse
Kod SKU
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