- Nucleoside analysis of DNA from Trypanosoma brucei and Trypanosoma equiperdum.
Nucleoside analysis of DNA from Trypanosoma brucei and Trypanosoma equiperdum.
Molecular and biochemical parasitology (1988-11-01)
M Crozatier, R J De Brij, L Den Engelse, P J Johnson, P Borst
PMID3185618
ABSTRAKT
We have digested trypanosome DNA with a combination of pancreatic DNase I, nuclease P1 and bovine alkaline phosphatase and fractionated the resulting nucleosides on a Supelcosil LC-18-S column by high pressure liquid chromatography. We find less than 0.1% unusual nucleosides, both in Trypanosoma brucei and in a Trypanosoma equiperdum stock, in contrast to a previous report of an unusual nucleoside replacing dC at 1.3% of total nucleosides in T. equiperdum. Our results agree with previous suggestions that the modification of inactive telomeric expression sites for variant-specific surface glycoprotein genes in T. brucei only affects a very small fraction of the total DNA.
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