G005826

LC/MS (TOF) Analysis of Dermorphin on Ascentis^® Express F5

application for HPLC

Właściwości

• metody: HPLC: suitable
• parametry testowe: column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U); mobile phase: 2 mM ammonium formate (90:10 acetonitrile:water) adjusted to pH 4.5 with formic acid; flow rate: 0.4 mL/min; column temp.: 35 °C; detector: ESI(+), TIC 100 – 1000 m/z; APCI (+), XIC m/z = 150.1, 203.7, 378.2; injection: 1 μL; sample: 10 μg/mL in 75:25 acetonitrile:water
• przydatność: application for HPLC
• Zastosowanie: forensics and toxicology

Opis

Komentarz do analizy

Dermorphin is a heptapeptide derived from frogs. It is a painkiller and used illicitly in racehorses so they continue to run through the pain. A rapid analysis of dermorphin standard is shown here on an Ascentis Express F5 column. The HILIC mode separation provided the necessary retention and MS-compatibility, while the pentafluorophenyl (F5) phase chemistry elimated the chelating interactions that are problematic on bare silica.

Informacje prawne

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Analit

Dermorphin trifluoroacetate salt, ≥98% (HPLC)