G000594

HPLC Analysis of Bronchodilators Theophylline and Other Caffeine Metabolites in Serum on Discovery^® RP-AmideC16 after SPE using Discovery^® DSC-18

application for SPE, application for HPLC

Właściwości

• metody: HPLC: suitable; solid phase extraction (SPE): suitable
• parametry testowe: sample preparation: SPE (Solid Phase Extraction); SPE tube/cartridge: Discovery^® DSC-18, 500 mg/3 mL (52603-U); condition: 2 mL methanol, then 2 mL water; sample preparation: 1 mL porcine serum spiked with 0.1 μg/mL, 0.50 μg/mL, or 1.0 μg/mL each analyte; sample preparation: 1 mL methanol; evaporate to dryness with nitrogen stream at room temperature; reconstitute in 200 μL mobile phase containing 0.2 μg/mL or 7-methylxanthine (IS); sample preparation: 2 mL 5% methanol in water; dry tube 10 min with nitrogen stream; column: Discovery RP-AmideC16, 15 cm × 4.6 mm, 5 μm particles, preceded by a 2 cm RP-AmideC16 guard column and 0.5 μm frit filter (505013); mobile phase: [A] Methanol; [B] 1% acetic acid (17:38, A:B); flow rate: 1 mL/min; column temp.: 30 °C; detector: UV, 272 nm; injection: 20 μL reconstituted porcine serum extract
• przydatność: application for HPLC; application for SPE
• Zastosowanie: clinical; pharmaceutical (small molecule)

Opis

Informacje prawne

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Analit

Caffeine
Theobromine
Theophylline
7-Methylxanthine, ≥98.0% (HPLC)
1,7-Dimethylxanthine, ~98%, solid