Przejdź do zawartości
Merck
Wszystkie zdjęcia(12)

Key Documents

ZRB1125

Sigma-Aldrich

Anti-LC3A Antibody, clone 3J12 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonim(y):

Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A/1B light chain 3A

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

rekombinowane

expressed in HEK 293 cells

białko sprzężone

unconjugated

forma przeciwciała

purified antibody

klon

recombinant monoclonal

linia produktu

ZooMAb® learn more

Postać

lyophilized

masa cząsteczkowa

calculated mol wt 14.27 kDa
observed mol wt ~14 kDa

reaktywność gatunkowa

human, mouse, rat

opakowanie

antibody small pack of 25 μL

charakterystyka ekologicznej alternatywy

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

rozszerzona walidacja

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

metody

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

izotyp

IgG

numer dostępu UniProt

kategoria ekologicznej alternatywy

Warunki transportu

ambient

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

Opis ogólny

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specyficzność

Clone 3J12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Autophagy-related protein LC3A. It targets an epitope within 25 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of human LC3A.

Zastosowanie

Quality Control Testing
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected LC3A in Rat Brain tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected LC3A in Human brain, mouse brain and PC12 lysates.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LC3A in SH-SY5Y cells.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected LC3A in human cerebral cortex tissue sections.Affinity Binding Assay: A representative lot of this antibody bound LC3A peptide with a KD of 3.3 x 10-8 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-LC3A, clone 3J12 ZooMAb, Cat. No. ZRB1125, is a recombinant Rabbit monoclonal antibody that specifically targetsLC3A and has been tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Opis wartości docelowych

Microtubule-associated proteins 1A/1B light chain 3A (UniProt: Q9H492; also known as Autophagy-related protein LC3A, Autophagy-related ubiquitin-like modifier LC3A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha) is encoded by the MAP1LC3A gene (Gene ID: 84557) in human. LC3s are structural proteins of autophagosomal membranes and are widely used as biomarkers of autophagy. They are ubiquitously distributed in all eukaryotes and are expressed as three splice variants known as LC3A, LC3B, and LC3C. LC3A is mainly localized to perinuclear and nuclear regions, LC3B is evenly distributed throughout the cytoplasm and is also localized in the nucleolar regions, and LC3C is shown to be located in the cytoplasm and strongly in the nuclei. Two variants (v1 and v2) of LC3A have been described that have different transcriptional start sites. Nuclear LC3A is shown to co-localize with Beclin-1. All three LC3s show a conserved region that is required for autophagosome formation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Koukourakis, MI., et al. (2015). PLoS ONE 10(9); e0137675).

Postać fizyczna

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstytucja

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informacje prawne

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej