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Key Documents

ZRB1116

Sigma-Aldrich

Anti-CD56 Antibody, clone 3F5 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonim(y):

Neural cell adhesion molecule 1;N-CAM-1;NCAM-1

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

rekombinowane

expressed in HEK 293 cells

białko sprzężone

unconjugated

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

3F5, recombinant monoclonal

opis

recombinant, expressed in HEK 293 cells

linia produktu

ZooMAb® learn more

Postać

lyophilized

masa cząsteczkowa

calculated mol wt 94.57 kDa
observed mol wt ~120 kDa

oczyszczone przez

using Protein A

reaktywność gatunkowa

human, rat

reaktywność gatunkowa (przewidywana na podstawie homologii)

bovine, monkey, mouse, feline, canine

opakowanie

antibody small pack of 25 μL

charakterystyka ekologicznej alternatywy

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

rozszerzona walidacja

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

metody

affinity binding assay: suitable
flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

izotyp

IgG

sekwencja epitopowa

N-terminal

numer dostępu Protein ID

numer dostępu UniProt

kategoria ekologicznej alternatywy

Warunki transportu

ambient

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

Opis ogólny

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specyficzność

Clone 3F5 is a Rabbit recombinant monoclonal antibody that specifically detects Neural cell adhesion molecule 1 (CD56). It targets an epitope within 23 amino acids from the N-terminal, extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal, extracellular domain of human Neural cell adhesion molecule 1 (CD56).

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CD56 in Rat brain tissue lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CD56 in human brain tissue lysate.

Affinity Binding Assay: A representative lot of this antibody bound CD56 with a KD of 1.5 x 10-9 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD56 (NCAM1) in human cerebral cortex tissue sections.

Flow Cytometry Analysis: 1 µg of a representative lot of this antibody detected CD56 in one million human PBMCs.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Opis wartości docelowych

Neural cell adhesion molecule 1 (UniProt: P13591; also known as N-CAM-1, NCAM-1, CD56) is encoded by the NCAM1 (also known as NCAM) gene (Gene ID: 4684) in human. CD56 is a member of the immunoglobulin superfamily that is engaged in both so-called homophilic and heterophilic interactions. It is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-718), a transmembrane domain (aa 719-739), and a cytoplasmic domain (aa 740-858). It also contains five Ig-like C2-type domains and two fibronectin type-III domains. CD56 is expressed by neurons, glia, skeletal muscle, and natural killer (NK) cells. It is involved in neuron-neuron adhesion, neurite fasciculation, and outgrowth of neurite. It is considered as the archetypal phenotypic marker for natural killer cells, but is also expressed in many other immune cells, including ab T cells, g T cells, dendritic cells, and monocytes. It is also shown to play a role in the fungus mediated NK cell activation and blocking of CD56 surface receptor reduced fungal mediated NK cell activation and reduced cytokine secretion. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ziegler, S., et al. (2017). Sci. Rep. 7; Article 6138; Van Acker, HH., et al. (2017). Front. Immunol. 8; Article 892).

Postać fizyczna

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstytucja

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informacje prawne

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

13 - Non Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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