Wszystkie zdjęcia(3)
Kluczowe dokumenty
V4139
Anti-Vinculin Antibody
rabbit polyclonal
Synonim(y):
Vinculin Antibody - Anti-Vinculin antibody produced in rabbit, Vinculin Antibody Sigma
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Polecane produkty
Nazwa produktu
Anti-Vinculin antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
pochodzenie biologiczne
rabbit
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
Formularz
buffered aqueous solution
masa cząsteczkowa
antigen ~116 kDa
reaktywność gatunkowa
rat, human, mouse
rozszerzona walidacja
independent
Learn more about Antibody Enhanced Validation
metody
indirect immunofluorescence: 15-30 μg/mL using acetone-fixed frozen sections of rat or human heart
western blot: 2.5-5 μg/mL using whole extracts of cultured mouse NIH3T3 cells and human hepatoma HepG2 cells
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)
Opis ogólny
In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletalmuscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.
Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.
Immunogen
synthetic peptide corresponding to amino acid residues 691-705 of human vinculin with N-terminal added cysteine, conjugated to KLH. This sequence is also present in metavinculin. The corresponding sequence is identical is mouse, rat, and pig and differs by one amino acid in chicken.
Zastosowanie
Anti-Vinculin antibody produced in rabbit has been used in immunoprecipitation.
Anti-vinculin antibody produced in rabbit has been used in following studies:
- immunofluorescence
- immuno-histology
- western blotting
Działania biochem./fizjol.
Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading. In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. Experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.
Vinculin also binds α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly. It transiently binds the actin-related protein-2/3 (ARP2/3) complex without colocalization in focal adhesions.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Ta strona może zawierać tekst przetłumaczony maszynowo.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
produkt powiązany
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Fibroblast expression of α-smooth muscle actin, α2β1 integrin and αvβ3 integrin: Influence of surface rigidity
Jones C and Ehrlich HP
Experimental and Molecular Pathology, 91, 394-399 (2011)
How vinculin regulates force transmission
Dumbauld DW, et al.
Proceedings of the National Academy of Sciences of the USA, 110(24), 9788-9793 (2013)
The differential effects of aligned electrospun PHBHHx fibers on adipogenic and osteogenic potential of MSCs through the regulation of PPARg signaling
Wang Y
Biomaterials, 33, 485-493 (2012)
Avital Gilam et al.
Nature communications, 7, 12868-12868 (2016-09-20)
Metastasis is the primary cause for mortality in breast cancer. MicroRNAs, gene expression master regulators, constitute an attractive candidate to control metastasis. Here we show that breast cancer metastasis can be prevented by miR-96 or miR-182 treatment, and decipher the
Sex-specific association of rs4746172 of VCL gene with hypertension in two Han populations from Southern China.
Yu Q
Scientific Reports (2015)
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej