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U1875

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

Type III, glycerol solution, 500-800 units/mL

Synonim(y):

Amidohydrolaza mocznikowa, Ureaza fasoli szparagowej

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About This Item

Numer CAS:
9002-13-5
Numer EC enzymu:
3.5.1.5 (BRENDA, IUBMB)
Numer MDL:
MFCD00070858
Kod UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

pochodzenie biologiczne

Canavalia ensiformis

Poziom jakości

200

typ

Type III

Formularz

glycerol solution

masa cząsteczkowa

~544620

stężenie

500-800 units/mL

temp. przechowywania

2-8°C

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Opis ogólny

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease of jack bean (Canavalia ensiformis), a hexamer of a single chain of 840 amino acid residues, is found in plants, bacteria, fungi, algae, and soil. It is considered the first nickel-containing enzyme and the first enzyme to be crystallized. Ureases exist in several isoforms, some are jack bean urease (JBURE-I), canatoxin (CNTX), and JBURE-IIB. JBURE-I is more abundant than the other two isoforms. Ureases are found in mature seeds.

Zastosowanie

Urease from Canavalia ensiformis (Jack bean) has been used:
  • to assay creatine and creatine phosphate using the sample extracted from the left ventricle (LV) and septal tissue
  • to determine the concentration of urea in a stabilized urine sample
  • as one of the standards to measure the urease activity of plant extracts

Działania biochem./fizjol.

In plants, ureases are essential for the bioavailability/metabolism of nitrogen in nature and defense mechanisms. Ureases are metalloenzymes that catalyze the hydrolysis of urea to release ammonia a nitrogen source for growth and carbon dioxide. They exhibit internal peptides-mediated insecticidal action and fungicidal activity.
Urease activity has been shown to be inhibited by benzpyrene following exposure to light. This inhibition was due to the effect of benzpyrene on urease′s sulfhydryl groups.

Definicja jednostki

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)
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Piktogramy

Health hazardExclamation mark
GHS08,GHS07

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H315,H319,H334,H335

Klasyfikacja zagrożeń

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
SLCK9718
SLCD8260
SLBX6511
SLBQ1942V
110M7352V

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Effect of light-activated benzpyrene on urease activity.
G C MILLS et al.
Cancer research, 13(1), 69-72 (1953-01-01)
Nizam Uddin et al.
Fitoterapia, 84, 202-207 (2012-10-30)
Seven constituents were isolated from the stems of Lawsonia alba Lam., following an activity-guided isolation, which include two new constituents, namely lawsorosemarinol (1) and lawsofructose (2), one known compound 2-(β-d-glucopyranosyloxy)-1, 4-naphthoquinone (3) and four compounds, 4-hydroxy coumarine (4), 3-(4-hyroxyphenyl)-triacontyl-(Z)-propenoate (5)
Brian H Eisner et al.
The Urologic clinics of North America, 40(1), 21-30 (2012-11-28)
This article reviews the data on pharmacologic treatment of kidney stone disease, with a focus on prophylaxis against stone recurrence. One of the most effective and important therapies for stone prevention, an increase in urine volume, is not discussed because
Yi Wen et al.
Journal of bacteriology, 195(3), 444-452 (2012-10-30)
Urease is an essential component of gastric acid acclimation by Helicobacter pylori. The increased level of urease in gastric acidity is due, in part, to acid activation of the two-component system consisting of the membrane sensor HP0165 (ArsS) and its
Lisa G Kuhns et al.
The Biochemical journal, 450(1), 141-148 (2012-11-28)
The persistence of the gastric pathogen Helicobacter pylori is due in part to urease and Msr (methionine sulfoxide reductase). Upon exposure to relatively mild (21% partial pressure of O2) oxidative stress, a Δmsr mutant showed both decreased urease specific activity
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