TP0200
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification
sufficient for ~50 manual assays
Synonim(y):
Zestaw do oznaczania białka całkowitego
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About This Item
Kod UNSPSC:
12352202
NACRES:
NA.32
Polecane produkty
opis
wavelength 500-750nm
Poziom jakości
zastosowanie
sufficient for ~50 manual assays
zakres pomiaru poziomu wykrywania białka
0-1 mg/mL
Parametry
18-26 °C temperature
200 μL sample volume
temp. przechowywania
2-8°C
Opis ogólny
The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.
Zastosowanie
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
- in the dialyzates/extracts of gliadins
- in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoa, Salvia hispanica L.)
- in the stored samples of rat liver microsomal fractions
Zasada
Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.
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Elementy zestawu są też dostępne oddzielnie
Numer produktu
Opis
Karta charakterystyki
- B3934Biuret reagent, protein detection level 150-1,000 μg/mL 110 mLKarta charakterystyki
- Folin & Ciocalteu’s phenol reagent 5 mL
wymagane, ale niedostarczone
Numer produktu
Opis
Cennik
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
Kod klasy składowania
8A - Combustible corrosive hazardous materials
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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