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T1192
Anti-Thymine Dimer antibody, Mouse monoclonal
clone H3, purified from hybridoma cell culture
Synonim(y):
Mouse Anti-Thymine Dimer, Thymine Dimer Detection, Thymine Dimer Mouse Antibody
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About This Item
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
H3, monoclonal
Postać
buffered aqueous solution
reaktywność gatunkowa
chicken, wide range
opakowanie
antibody small pack of 25 μL
stężenie
~2 mg/mL
metody
capture ELISA: suitable
dot blot: 0.5-1 μg/mL
immunocytochemistry: suitable
izotyp
IgG1
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
Opis ogólny
UV light induces the formation of thymine dimers that subsequently leads to DNA damage in cells. Monoclonal antibodies that bind to thymine dimers enable the study of cell death and UV-induced DNA damage during photocarcinogenesis . Monoclonal Anti-Thymine Dimer binds to the (5′-6′) cyclobutane type of homothymine or thyminecytosine heterodimers in single-stranded DNA. The antibody has a decreased affinity for the dimer in short oligonucleotides (an oligonucleotide of atleast 10-20 thymine residues is required for the labeling of the probes). The product facilitates the use of a sensitive and non-radioactive technique for the analysis of thymine dimerization.
Immunogen
tetra nucleotide containing cyclobutane thymine dimer (GTTG) conjugated to chicken gamma globulin.
Zastosowanie
May be used in Southern blotting at 0.5-1μg/mL using UV induced thymine dimers of λ phage DNA fragments.
Monoclonal Anti-Thymine Dimer antibody is suitable for use in dot blot (0.5-1 mg/mL), immunocytochemistry (1:100) and capture ELISA.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Inne uwagi
Distributed under license.
Patents WO87/01134, EP 0233 177 B1
Patents WO87/01134, EP 0233 177 B1
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
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Melanin is a free-radical scavenger, antioxidant, and broadband absorber of ultraviolet (UV) radiation which protects the skin from environmental carcinogenesis. However, melanin synthesis and UV-induced reactive melanin species are also implicated in melanocyte genotoxicity. Here, we attempted to reconcile these
Detection of cyclobutane thymine dimers in DNA of human cells with monoclonal antibodies raised against a thymine dimer-containing tetranucleotide.
L Roza et al.
Photochemistry and photobiology, 48(5), 627-633 (1988-11-01)
O J Marvik et al.
BioTechniques, 23(5), 892-896 (1998-02-12)
We describe a simple detection system for DNA based on antibody detection of UV-induced photoproducts. It includes a convenient and inexpensive labeling procedure, which is completed in 5-10 min. The only equipment required is a UV source such as an
H H Slagsvold et al.
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In cerebellar granule cells a rapid necrotic cell death has been observed during and immediately after glutamate exposure, followed by a delayed apoptotic type of neuronal death in a subpopulation of the surviving neurons. In some experimental models the DNA
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