SRP0258

PDE1B Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Synonim(y): Calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase, Cam-PDE 63 kDa, PDES1B, Phosphodiesterase 1B
• Kod UNSPSC: 12352200
• NACRES: NA.32

Właściwości

• pochodzenie biologiczne: human
• rekombinowane: expressed in baculovirus infected insect cells
• Próba: ≥70% (SDS-PAGE)
• Postać: aqueous solution
• aktywność właściwa: ≥2000 units/μg
• masa cząsteczkowa: 87 kDa
• opakowanie: pkg of 10 μg
• stężenie: >0.02 mg/mL
• numer dostępu NCBI: NM_000924
• numer dostępu UniProt: Q01064
• Warunki transportu: dry ice
• temp. przechowywania: −70°C
• informacje o genach: human ... PDE1B(5153)

Opis

Opis ogólny

Human PDE1B (GenBank Accession No. NM_000924), full length with N-terminal GST tag, MW = 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Zastosowanie

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definicja jednostki

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

Postać fizyczna

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Uwaga dotycząca przygotowania

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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