SAB4702137

Monoclonal Anti-CD3 PE-Cyanine7 produced in mouse

• Synonim(y): CD3E, T3E, TCRE
• Kod UNSPSC: 12352203

Właściwości

• białko sprzężone: PE-Cy7 conjugate
• Poziom jakości: 100
• rodzaj przeciwciała: primary antibodies
• klon: SK7, monoclonal
• Postać: buffered aqueous solution
• reaktywność gatunkowa: ( Human), (Non-human primates)
• metody: flow cytometry: suitable
• izotyp: IgG1κ
• numer dostępu UniProt: P07766
• temp. przechowywania: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... CD3E(916)

Opis

Opis ogólny

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Specyficzność

The mouse monoclonal antibody SK7 recognizes an extracellular epitope on the CD3 antigen on mature human T cells. This antibody reacts with the epsilon chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes.

Immunogen

Human thymocytes

Postać fizyczna

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Uwaga dotycząca przygotowania

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Przechowywanie i stabilność

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Inne uwagi

The antibody solution should be gently mixed before use.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable