Wszystkie zdjęcia(3)
Kluczowe dokumenty
SAB4200810
Anti-Podocin, Mouse monoclonal
clone PD-65, purified from hybridoma cell culture
Synonim(y):
NPHS2
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352203
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
forma przeciwciała
purified from hybridoma cell culture
rodzaj przeciwciała
primary antibodies
klon
PD-65, monoclonal
Formularz
buffered aqueous solution
masa cząsteczkowa
~42 kDa
reaktywność gatunkowa
bovine, rat, human, mouse
opakowanie
antibody small pack of 25 μL
stężenie
1 mg/mL
metody
immunoblotting: 0.25-0.5 μg/mL using using human HEK-293T cells.
immunofluorescence: 1-2 μg/mL using human HEK-293T cells.
izotyp
IgG2a
numer dostępu UniProt
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... NPHS2(7827)
Opis ogólny
Podocytes are highly specialized visceral epithelial cells that cover the outer aspect of the glomerulus in the kidney. The foot processes of adjacent podocytes regularly interdigitate leaving filtration slits that are bridged by a thin slit diaphragm. This is an extracellular adherens-like junction that serves as a barrier to macromolecules and plays a crucial role in the glomerular filtration process. Podocin (NPHS2 protein) is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band-7-stomatin family of lipid raft-associated proteins. It is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It interacts there with nephrin (NPHS1 protein), a protein critical in development and function of the kidney filtration barrier and the adapter protein CD2-AP. Podocin is anchored in the glycosphingolipids and cholesterol rich lipid rafts of the outer leaflet of the plasma membrane and is partially localized with the actin cytoskeleton. In vitro studies suggest its involvement in nephrin signaling facilitation via AP-1 in HEK cells. In addition to its presence in the kidney, it was also reported in rat nervous tissue and possibly in vascular smooth muscle.
Immunogen
Synthetic peptide of human Podocin , conjugated to KLH
Zastosowanie
The antibody may be used in various immunochemical techniques including Immunobloting (~42kDa) and Immunofluorescence. Detection of the Podocin band by Immunoblotting is specifically inhibited by the immunogen.a
Postać fizyczna
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative
Inne uwagi
This product is for R&D use only, not for drug, household, or other uses.
Ta strona może zawierać tekst przetłumaczony maszynowo.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Wybierz jedną z najnowszych wersji:
Certyfikaty analizy (CoA)
Lot/Batch Number
Nie widzisz odpowiedniej wersji?
Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Hiroshi Kawachi et al.
Journal of the American Society of Nephrology : JASN, 14(1), 46-56 (2002-12-31)
Podocin is identified as a product of the gene mutated in a patient with autosomal recessive steroid-resistant nephrotic syndrome. Although podocin is reported to be located at the slit diaphragm area, the precise role of podocin for maintaining the barrier
Séverine Roselli et al.
Traffic (Copenhagen, Denmark), 5(1), 37-44 (2003-12-17)
Podocytes are specialized epithelial cells of the glomerulus in the kidney, which interconnect at the top of the glomerular basement membrane through the slit diaphragm, an adherens-like junction that plays a crucial role in the glomerular filtration process. Podocin, a
Izumi Horinouchi et al.
Kidney international, 64(6), 2092-2099 (2003-11-25)
Mutations of the NPHS2 gene are responsible for autosomal-recessive steroid-resistant nephrotic syndrome. Its product, podocin, faces the slit diaphragm area with its two ends in the cytoplasm of foot processes. We generated rabbit polyclonal antibodies against conjugated peptides from human
G Caridi et al.
Journal of the American Society of Nephrology : JASN, 12(12), 2742-2746 (2001-12-01)
Podocin mutations (NPHS2 gene) are responsible for the autosomal recessive form of steroid-resistant nephrotic syndrome. As a result of a screening for these gene alterations in a cohort of Italian patients with nonfamilial nephrotic syndrome and histologic focal segmental glomerulosclerosis
Séverine Roselli et al.
Molecular and cellular biology, 24(2), 550-560 (2004-01-01)
Podocytes are specialized epithelial cells covering the basement membrane of the glomerulus in the kidney. The molecular mechanisms underlying the role of podocytes in glomerular filtration are still largely unknown. We generated podocin-deficient (Nphs2-/-) mice to investigate the function of
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej