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SAB4200753
Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal
clone AK-11, purified from hybridoma cell culture
Synonim(y):
Anti-Protein Kinase B, Anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.44
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified from hybridoma cell culture
rodzaj przeciwciała
primary antibodies
klon
AK-11, monoclonal
Postać
buffered aqueous solution
masa cząsteczkowa
~56 kDa
reaktywność gatunkowa
mouse, chicken, rat, human
stężenie
~1.0 mg/mL
metody
immunoblotting: 1-2 μg/mL using human breast cancer cell line MCF-7 extract
immunofluorescence: 1-2 μg/mL using mouse embryo fibroblast NIH-3T3 cells
immunoprecipitation (IP): 2-4 μg/test using whole extract of mouse embryo fibroblast NIH-3T3 cell line
izotyp
IgG2a
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
phosphorylation (pThr450)
informacje o genach
human ... AKT1(207)
Opis ogólny
Anti-phospho-AKT (pThr450) antibody, mouse monoclonal (mouse IgG2a isotype) is derived from the AK-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Serine/threonine kinase (AKT) also known as protein kinase B (PKB) belongs to the family of serine/threonine kinases. It consists of three isoforms such as AKT1, AKT2 and AKT3. AKT1 gene is located on the human chromosome at 14q32.33.
Specyficzność
Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal specifically recognizes all three AKT isoforms phosphorylated at Thr450 (AKT1, AKT2 and AKT3).
Immunogen
Synthetic peptide corresponding to human AKT1 pThr450
Zastosowanie
Anti-phospho-AKT (pThr450) antibody, mouse monoclonal may be used in:
- immunoblotting
- immunofluorescence
- immunoprecipitation
Działania biochem./fizjol.
Serine/threonine kinase (AKT) plays an important role in the control of cell cycle and cell proliferation. It is also involved in cell differentiation and apoptosis signaling pathways. AKT is rapidly activated in response to cell stimulation by several growth factors, such as insulin, peroxyvanadate or cellular stresses (such as heat shock). Following activation, the phosphorylated AKT is relocated to the nucleus. Lack of phosphorylation at Thr450 enhances AKT ubiquitination and degradation.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Przechowywanie i stabilność
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
H Dudek et al.
Science (New York, N.Y.), 275(5300), 661-665 (1997-01-31)
A signaling pathway was delineated by which insulin-like growth factor 1 (IGF-1) promotes the survival of cerebellar neurons. IGF-1 activation of phosphoinositide 3-kinase (PI3-K) triggered the activation of two protein kinases, the serine-threonine kinase Akt and the p70 ribosomal protein
Zhi-Ping Yang et al.
World journal of gastroenterology, 21(36), 10385-10399 (2015-10-01)
To investigate the anti-tumor effects of equol in gastric cancer cells and the underlying molecular mechanisms. MGC-803 cells were employed for in vitro experiments in this study. Cells were treated with control (vehicle, 0.1% DMSO) or equol under specified dose
Won Jun Oh et al.
The EMBO journal, 29(23), 3939-3951 (2010-11-04)
The mechanisms that couple translation and protein processing are poorly understood in higher eukaryotes. Although mammalian target of rapamycin (mTOR) complex 1 (mTORC1) controls translation initiation, the function of mTORC2 in protein synthesis remains to be defined. In this study
A Khwaja et al.
The EMBO journal, 16(10), 2783-2793 (1997-05-15)
Upon detachment from the extracellular matrix, epithelial cells enter into programmed cell death, a phenomenon known as anoikis, ensuring that they are unable to survive in an inappropriate location. Activated ras oncogenes protect cells from this form of apoptosis. The
L Xiao et al.
Cell death and differentiation, 17(9), 1448-1462 (2010-02-27)
AKT pathway has a critical role in mediating signaling transductions for cell proliferation, differentiation and survival. Previous studies have shown that AKT activation is achieved through a series of phosphorylation steps: first, AKT is phosphorylated at Thr-450 by JNK kinases
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