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SAB4200248
Anti-Actin antibody, Mouse monoclonal
clone MM2/193, purified from hybridoma cell culture
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About This Item
pochodzenie biologiczne
mouse
białko sprzężone
unconjugated
forma przeciwciała
purified from hybridoma cell culture
rodzaj przeciwciała
primary antibodies
klon
MM2/193, monoclonal
Postać
buffered aqueous solution
masa cząsteczkowa
antigen ~42 kDa
reaktywność gatunkowa
mouse, human, rat, canine, monkey
stężenie
~1.0 mg/mL
metody
western blot: 1-2 μg/mL using HeLa cell extracts
izotyp
IgM
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... ACTB(60)
Opis ogólny
Monoclonal anti-actin (mouse IgM isotype) is derived from the hybridoma MM2/193 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes. Monoclonal anti-actin recognizes human, monkey, dog, rat and mouse actin (not tested in other species).
In mammals, actin protein consists of six different isoforms encoded by separate genes. The four isoforms αskeletal-actin, α cardiac-actin, αsmooth-actin and γsmooth-actin are expressed in skeletal, cardiac and smooth muscle. The other two isoforms, β cyto-actin and γ cyto-actin are ubiquitously expressed.
In mammals, actin protein consists of six different isoforms encoded by separate genes. The four isoforms αskeletal-actin, α cardiac-actin, αsmooth-actin and γsmooth-actin are expressed in skeletal, cardiac and smooth muscle. The other two isoforms, β cyto-actin and γ cyto-actin are ubiquitously expressed.
Immunogen
hybridoma MM2/193 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes
Zastosowanie
Monoclonal Anti-Actin antibody produced in mouse has been used in immunoblotting.
Działania biochem./fizjol.
Actin plays a vital role in various cellular processes, such as cell division, migration, junction formation, chromatin remodeling, transcriptional regulation, vesicle trafficking and cell shape regulation. Polymorphism in the smooth muscle alpha-actin (ACTA2) is associated with the development of thoracic aortic aneurysms and dissections.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Przechowywanie i stabilność
Store at -20°C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Inne uwagi
In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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