Wszystkie zdjęcia(1)
Key Documents
SAB4200237
Anti-Glutathione-S-transferase (GST) antibody, Mouse monoclonal
clone 2H3-D10, purified from hybridoma cell culture
Synonim(y):
Mouse Anti-GST Tag
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Polecane produkty
pochodzenie biologiczne
mouse
białko sprzężone
unconjugated
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
2H3-D10, monoclonal
Postać
buffered aqueous solution
stężenie
~1.0 mg/mL
metody
western blot: 0.2-0.4 μg/mL using detection limit for GST-fustion protein is ∼ 10ng/lane
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
Opis ogólny
Anti-Glutathione-S-transferase (GST) antibody, mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 2H3-D10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a GST-fusion protein. GST is a Phase II metabolic enzyme, expressed ubiquitously in both eukaryotes and prokaryotes. GSTs are classified in to three types based on their cellular localization, namely cytosolic GSTs, mitochondrial GSTs and microsomal GSTs.
Specyficzność
Monoclonal Anti- Glutathione-S-transferase (GST) recognizes GST from Schistosoma japonicum in GSTfusion proteins.
Immunogen
hybridoma 2H3-D10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a GST-fusion protein
Zastosowanie
Anti-Glutathione-S-transferase (GST) antibody, Mouse monoclonal has been used in immunoblotting.
Działania biochem./fizjol.
Glutathione-S-Transferase (GST) is an enzyme, which catalyzes the reaction of glutathione with a wide range of electrophilic substrates and therefore, plays a role in the detoxification of potential alkylating agents.(5) GST protects the cell against oxidative stress and several toxic molecules. It facilitates the synthesis and modification of leukotrienes and prostaglandins. GST from Schistosoma japonicum, has been cloned in several expression vectors as a 27.5 kDa molecule and used as a tag for expressing proteins of interest. These proteins are thus expressed as GST-fusion proteins. Antibodies to GST are useful in identifying the successful expression of these fusion proteins.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Przechowywanie i stabilność
Store at −20 °C. For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at 20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
polecane
Kod klasy składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Glutathione transferases: substrates, inihibitors and pro-drugs in cancer and neurodegenerative diseases
Allocati N, et al.
Oncogenesis, 7(1), 1-15 (2018)
HnRNPK/miR-223/FBXW7 feedback cascade promotes pancreatic cancer cell growth and invasion
De He CH, et al.
Testing, 8(12), 20165-20165 (2017)
Gabriele Zaffagnini et al.
The EMBO journal, 37(5) (2018-01-19)
The removal of misfolded, ubiquitinated proteins is an essential part of the protein quality control. The ubiquitin-proteasome system (UPS) and autophagy are two interconnected pathways that mediate the degradation of such proteins. During autophagy, ubiquitinated proteins are clustered in a
Audrey W Hong et al.
Bio-protocol, 7(14) (2017-11-07)
This protocol describes a method to directly measure LATS activity by an in vitro kinase assay using YAP as a substrate.
Phospholipase Dε interacts with autophagy-related protein 8 and promotes autophagy in Arabidopsis response to nitrogen deficiency.
Shuaibing Yao et al.
The Plant journal : for cell and molecular biology, 109(6), 1519-1534 (2021-12-25)
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej