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Key Documents

S2014

Sigma-Aldrich

Staphylococcus aureus

buffered aqueous suspension, Wood 46 strain

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About This Item

Kod UNSPSC:
12352202
NACRES:
NA.81

pochodzenie biologiczne

Staphylococcus aureus

Poziom jakości

200

sterylność

Not processed or packaged aseptically

Postać

buffered aqueous suspension

skład

Cell suspension, ~10% wet weight/volume

temp. przechowywania

2-8°C

Opis ogólny

Staphylococcus aureus Wood 46 is protein A deficient and spa negative. It shares 98% to 99% genome identity with S.aureus and shows a lower surface expression of cell wall-associated protein A.

Zastosowanie

(Not intended for use as a starter culture.)
Staphylococcus aureus has been used:
  • to mimic infection and induce fever in Pekin duck
  • to test its effect on hemocyte morphology in hemolymph samples from beetle Tenebrio molitor larva
  • in the antibacterial activity and minimum inhibitory concentration (MIC) assay with gedunin and 7-deacetoxy-7αhydroxygedunin potassium salt

Wood 46, a non-protein A producing S. aureus strain, prepared by the same method as P7155 (Protein A, crude cell suspension-Cowan strain), may be used as a control in protein A-immunoglobulin binding studies.

Działania biochem./fizjol.

Staphylococcus aureus Wood 46 displays reduced virulence compared to the S. aureus. This isolate is useful in understanding protein A role in pathogenesis and virulence.

Postać fizyczna

Formalin-fixed crude cell suspension of essentially non-viable S. aureus (Wood 46 strain) in 0.05 M potassium phosphate buffer, pH 7.5, containing 0.2% sodium azide

Uwaga dotycząca przygotowania

Produced in pure culture.

Komentarz do analizy

This strain binds less than 10% of the rabbit IgG bound by P 7155 as assayed by a modification of the method of Kessler.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

S W Kessler
Journal of immunology (Baltimore, Md. : 1950), 117(5 Pt 1), 1482-1490 (1976-11-01)
Procedures are detailed for the rapid isolation of representative cell membrane antigens with protein A-bearing staphylococci as an adsorbent for IgG antibodies complexed with the antigens. Cell surface membrane proteins were radioiodinated and solubilized in nonionic detergent. Specific antisera were
G Kronvall et al.
Infection and immunity, 3(1), 10-15 (1971-01-01)
Protein A of Staphylococcus aureus can be detected on cell walls of intact bacteria by use of radioactively labeled myeloma globulin. Of 156 strains of S. aureus, 141 (90%) contained protein A. None of 47 S. epidermidis strains was positive
M Marais et al.
Poultry science, 90(6), 1234-1238 (2011-05-21)
Poultry, like mammals and other birds, develop fever when exposed to compounds from gram-negative bacteria. Mammals also develop fever when exposed to the constituents of viruses or gram-positive bacteria, and the fevers stimulated by these different pathogenic classes have discrete
Elżbieta Czarniewska et al.
Scientific reports, 9(1), 14027-14027 (2019-10-03)
To induce the water solubility of hexagonal boron nitride (h-BN), we exfoliated and functionalized bulk h-BN with hydroxyl groups (h-BN-OH-n). Short-term studies showed that h-BN-OH-n induced low cytotoxicity in different models: insect haemocytes (in vivo), human erythrocytes and mouse fibroblasts
Arkadiusz Urbański et al.
Micron (Oxford, England : 1993), 104, 8-20 (2017-10-20)
The evolutionary success of insects is undoubtedly related to a well-functioning immune system. This is especially apparent during insect development by the adaptation of individuals to the changing risk of infection. In addition, current studies show that the insect immune
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