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P5400

Sigma-Aldrich

Pectinesterase from orange peel

greener alternative

lyophilized powder, ≥150 units/mg protein

Synonim(y):

Pectin methylesterase, Pectin pectylhydrolase

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About This Item

Numer CAS:
9025-98-3
Numer EC enzymu:
3.1.1.11 (BRENDA, IUBMB)
Numer WE:
232-807-0
Numer MDL:
MFCD00131812
Kod UNSPSC:
12352204
NACRES:
NA.54

Postać

lyophilized powder

Poziom jakości

200

aktywność właściwa

≥150 units/mg protein

skład

Protein, 20-50% biuret

charakterystyka ekologicznej alternatywy

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

kategoria ekologicznej alternatywy

, Enabling

temp. przechowywania

2-8°C

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Opis ogólny

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Zastosowanie

Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH4)2SO4 and sodium chloride. It has been used to study the pectin methylesterase gene.
The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.

Działania biochem./fizjol.

Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.

Definicja jednostki

One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.

Postać fizyczna

Contains (NH4)2SO4 and sodium chloride

Komentarz do analizy

Protein determined by biuret.

Inne uwagi

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
This page may contain text that has been machine translated.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikaty analizy (CoA)

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Kerstin Müller et al.
Plant physiology, 161(1), 305-316 (2012-11-07)
The methylesterification status of cell wall homogalacturonans, mediated through the action of pectin methylesterases (PMEs), influences the biophysical properties of plant cell walls such as elasticity and porosity, important parameters for cell elongation and water uptake. The completion of seed
Sergio T de Freitas et al.
The Plant journal : for cell and molecular biology, 71(5), 824-835 (2012-05-09)
Blossom-end rot (BER) in tomato fruit (Solanum lycopersicum) is believed to be a calcium (Ca²⁺) deficiency disorder, but the mechanisms involved in its development are poorly understood. Our hypothesis is that high expression of pectin methylesterases (PMEs) increases Ca²⁺ bound
Yuan Zhou et al.
Phytochemistry, 76, 46-51 (2012-01-11)
Aluminum (Al) toxicity is one of the most widespread problems for crop production on acid soils, and nitric oxide (NO) is a key signaling molecule involved in the mediation of various biotic and abiotic stresses in plants. Here we found
Yuri L Dorokhov et al.
PLoS pathogens, 8(4), e1002640-e1002640 (2012-04-13)
Many plants release airborne volatile compounds in response to wounding due to pathogenic assault. These compounds serve as plant defenses and are involved in plant signaling. Here, we study the effects of pectin methylesterase (PME)-generated methanol release from wounded plants
Doungla E Ngouémazong et al.
Carbohydrate research, 348, 69-76 (2012-01-03)
The influence of the degree and pattern of methylesterification (DM and PM, respectively) on the stiffness of Ca(2+)-pectin gels is extensively examined, at various Ca(2+) concentrations. Accordingly, a highly methyl-esterified pectin was selectively de-esterified using NaOH, plant or fungal pectin
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Produkty

Carbohydrate Analysis

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

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