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Key Documents

L8146

Sigma-Aldrich

Lectin from Ulex europaeus (gorse, furze)

peroxidase conjugate, lyophilized powder

Synonim(y):

Ulex europaeus agglutinin, UEA-I

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About This Item

Kod UNSPSC:
12352202
NACRES:
NA.32

pochodzenie biologiczne

Ulex europaeus

Poziom jakości

200

białko sprzężone

peroxidase conjugate

Próba

70.00-90.00% (Lowry)

Postać

lyophilized powder

siła działania

<40 μg/mL agglutination activity

peroxidase activity

≥15 units/mg protein

skład

Protein, ~80% Lowry

metody

western blot: suitable

temp. przechowywania

2-8°C

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Zastosowanie

General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Działania biochem./fizjol.

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Opakowanie

Package size based on protein content.

Powiązanie

Conjugates are prepared from affinity purified lectin.

Definicja jednostki

One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.

Postać fizyczna

Contains sodium citrate buffer salts

Uwaga dotycząca przygotowania

Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.
Repurified by affinity chromatography after conjugation.

Komentarz do analizy

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.
S Avrameas et al.
Immunochemistry, 8(12), 1175-1179 (1971-12-01)
F Bucardo et al.
Zoonoses and public health, 63(8), 600-607 (2016-05-14)
Information about porcine norovirus (PoNoV), genetically similar to human NoV (HuNoV), is limited from rural areas where household-raised pigs are heavily exposed to faecal material which could facilitate transmission. Histo-blood group antigens (HBGAs) are known susceptibility factors to NoV in
K Nozaki et al.
Journal of neurology, neurosurgery, and psychiatry, 80(8), 904-908 (2009-02-26)
To study the clinical and pathological correlations of neuromuscular patients with a high aldolase and normal creatine kinase (CK) in serum at presentation or during a symptomatic exacerbation. Records and muscle biopsies were retrospectively reviewed in a consecutive series of
Xiaohu Fan et al.
Human gene therapy, 21(7), 877-890 (2010-02-19)
The ABO histo-blood group system is the most important antigen system in transplantation medicine, yet no small animal model of the ABO system exists. To determine the feasibility of developing a murine model, we previously subcloned the human alpha-1,2-fucosyltransferase (H-transferase
J M Sweeter et al.
Scientific reports, 11(1), 13037-13037 (2021-06-24)
Exacerbations of muco-obstructive airway diseases such as COPD and asthma are associated with epithelial changes termed mucous metaplasia (MM). Many molecular pathways triggering MM have been identified; however, the factors that regulate resolution are less well understood. We hypothesized that
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