L8146
Lectin from Ulex europaeus (gorse, furze)
peroxidase conjugate, lyophilized powder
Synonim(y):
Ulex europaeus agglutinin, UEA-I
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About This Item
Kod UNSPSC:
12352202
NACRES:
NA.32
Polecane produkty
pochodzenie biologiczne
Ulex europaeus
Poziom jakości
białko sprzężone
peroxidase conjugate
Próba
70.00-90.00% (Lowry)
Formularz
lyophilized powder
siła działania
<40 μg/mL agglutination activity
peroxidase activity
≥15 units/mg protein
skład
Protein, ~80% Lowry
metody
western blot: suitable
temp. przechowywania
2-8°C
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Zastosowanie
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Działania biochem./fizjol.
Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
Opakowanie
Package size based on protein content.
Powiązanie
Conjugates are prepared from affinity purified lectin.
Definicja jednostki
One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
Postać fizyczna
Contains sodium citrate buffer salts
Uwaga dotycząca przygotowania
Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.
Repurified by affinity chromatography after conjugation.
Repurified by affinity chromatography after conjugation.
Komentarz do analizy
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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