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Key Documents
L2380
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
lyophilized powder
Synonim(y):
Bandeirea simplicifolia agglutinin, BS-I
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About This Item
Kod UNSPSC:
12352202
NACRES:
NA.32
Polecane produkty
Postać
lyophilized powder
Poziom jakości
siła działania
<20 μg per mL agglutination activity (using human blood group B erythrocytes)
skład
Protein, ≥90% biuret
zanieczyszczenia
salt, free
temp. przechowywania
2-8°C
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Działania biochem./fizjol.
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Inne uwagi
BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Uwaga dotycząca przygotowania
Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).
Komentarz do analizy
Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
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